ELISA/assay

Chymase 1, Mast Cell (CMA1) ELISA Kit

MBS2021042

48-Strip-Wells

565 USD

ELISA Kit

Chymase 1, Mast Cell (CMA1) ELISA Kit

Chymase 1, Mast Cell (CMA1)

CYH; CYM; Alpha-chymase; Mast cell protease I

CMA1

Human

This assay has high sensitivity and excellent specificity for detection of CMA1. No significant cross-reactivity or interference between CMA1 and analogues was observed.

For unopened kit: All the reagents should be kept according to the labels on vials. The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 degree C upon receipt while the others should be at 4 degree C. For opened kit: When the kit is opened, the remaining reagents still need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and reseal along entire edge of zip-seal. The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Samples: Human serum, plasma, tissue homogenates, cell lysates and other biological fluids. Assay Type: Sandwich. Detection Range: 31.2-2,000pg/mL. The standard curve concentrations used for the ELISA's were 2,000pg/mL, 1,000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL. Sensitivity: Typically less than 12.5pg/mL

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level CMA1 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level CMA1 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%

Intended Uses The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of CMA1 in human serum, plasma, tissue homogenates, cell lysates and other biological fluids. Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to CMA1. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to CMA1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain CMA1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of CMA1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

48-Strip-Wells

565 USD

96-Strip-Wells

765

5x96-Strip-Wells

2975

10x96-Strip-Wells

5340