Hepatocyte Growth Factor Activator (HGFAC) ELISA Kit | MyBioSource MBS2021005 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Hepatocyte Growth Factor Activator (HGFAC) ELISA Kit

catalog :

MBS2021005

quantity :

48-Strip-Wells

price :

565 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS2021005

products type :

ELISA Kit

products full name :

Hepatocyte Growth Factor Activator (HGFAC) ELISA Kit

products short name :

Hepatocyte Growth Factor Activator (HGFAC)

products name syn :

HGFA; HGFAP; HGF Activator

other names :

hepatocyte growth factor activator isoform 2 preproprotein; Hepatocyte growth factor activator; hepatocyte growth factor activator; HGF activator

products gene name :

HGFAC

other gene names :

HGFAC; HGFAC; HGFA; HGF activator; HGFA

uniprot entry name :

HGFA_HUMAN

reactivity :

Human

sequence length :

655

specificity :

This assay has high sensitivity and excellent specificity for detection of Hepatocyte Growth Factor Activator (HGFAC). No significant cross-reactivity or interference between Hepatocyte Growth Factor Activator (HGFAC) and analogues was observed.

storage stability :

For unopened kit: All the reagents should be kept according to the labels on vials. The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 degree C upon receipt while the others should be at 4 degree C. For opened kit: When the kit is opened, the remaining reagents still need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and reseal along entire edge of zip-seal. The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

other info1 :

Samples: Serum, plasma, tissue homogenates and other biological fluids. Assay Type: Sandwich. Detection Range: 15.63-1000pg/mL. Sensitivity: Typically less than 6.7pg/mL.

other info2 :

Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Hepatocyte Growth Factor Activator (HGFAC) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Hepatocyte Growth Factor Activator (HGFAC) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 . Intra-Assay: CV<10% . Inter-Assay: CV<12% . Assay Procedure Summary: 1. Prepare all reagents, samples and standards;. 2. Add 100uL standard or sample to each well. Incubate 2 hours at 37 degree C;. 3. Aspirate and add 100uL prepared Detection Reagent A. Incubate 1 hour at 37 degree C;. 4. Aspirate and wash 3 times;. 5. Add 100uL prepared Detection Reagent B. Incubate 30 minutes at 37 degree C;. 6. Aspirate and wash 5 times;. 7. Add 90uL Substrate Solution. Incubate 15-25 minutes at 37 degree C;. 8. Add 50uL Stop Solution. Read at 450nm immediately.

products description :

Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Hepatocyte Growth Factor Activator (HGFAC). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Hepatocyte Growth Factor Activator (HGFAC). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Hepatocyte Growth Factor Activator (HGFAC), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Hepatocyte Growth Factor Activator (HGFAC) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

ncbi gi num :

4504383

ncbi acc num :

NP_001519.1

ncbi gb acc num :

NM_001528.3

ncbi mol weight :

70,682 Da

ncbi summary :

This gene encodes a member of the peptidase S1 protein family. The encoded protein is first synthesized as an inactive single-chain precursor before being activated to a heterodimeric form by endoproteolytic processing. It acts as serine protease that converts hepatocyte growth factor to the active form. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2014]

uniprot summary :

HGFAC: Activates hepatocyte growth factor (HGF) by converting it from a single chain to a heterodimeric form. Belongs to the peptidase S1 family. Protein type: Secreted; Protease; Secreted, signal peptide; EC 3.4.21.-. Chromosomal Location of Human Ortholog: 4p16. Cellular Component: extracellular space; extracellular region. Molecular Function: serine-type peptidase activity; serine-type endopeptidase activity. Biological Process: proteolysis

size1 :

48-Strip-Wells

price1 :

565 USD

size2 :

96-Strip-Wells

price2 :

765

size3 :

5x96-Strip-Wells

price3 :

2975

size4 :

10x96-Strip-Wells

price4 :

5340

more info or order :

MyBioSource product webpage