ELISA/assay

Fibroblast Growth Factor 18 (FGF18) ELISA Kit

MBS2020995

24-Strip-Wells

270 USD

ELISA Kit

Fibroblast Growth Factor 18 (FGF18) ELISA Kit

[Fibroblast Growth Factor 18 (FGF18)]

[ZFGF5]

[fibroblast growth factor 18; Fibroblast growth factor 18; fibroblast growth factor 18; fibroblast growth factor 18; zFGF5]

[FGF18]

[FGF18; FGF18; ZFGF5; FGF-18; FGF-18]

FGF18_HUMAN

Mouse

207

This assay has high sensitivity and excellent specificity for detection of Fibroblast Growth Factor 18 (FGF18). No significant cross-reactivity or interference between Fibroblast Growth Factor 18 (FGF18) and analogues was observed.

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Standard Curve/Testing Data

Assay Type: Double-antibody Sandwich. Samples: Serum, Plasma, Tissue homogenates, Cell lysates, Cell culture supernates and Other Biological Fluids. Detection Range: 15.6-1000pg/mL. Sensitivity: < 6.5pg/mL

Application: Enzyme-linked immunosorbent assay for Antigen Detection. Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Fibroblast Growth Factor 18 (FGF18) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Fibroblast Growth Factor 18 (FGF18) were tested on 3 different plates, 8 replicates in each plate. CV(%) =: SD/meanX100. Intra-Assay: CV<10%. Inter-Assay: CV<12%

Cytokine

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Fibroblast Growth Factor 18 (FGF18). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Fibroblast Growth Factor 18 (FGF18). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Fibroblast Growth Factor 18 (FGF18), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Fibroblast Growth Factor 18 (FGF18) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

4503695

NP_003853.1

NM_003862.2

O76093

23,989 Da

Activated Point Mutants Of FGFR2 Pathway (645281); Adaptive Immune System Pathway (366160); Constitutive PI3K/AKT Signaling In Cancer Pathway (685535); DAP12 Interactions Pathway (685549); DAP12 Signaling Pathway (685550); Disease Pathway (530764); Downstream Signal Transduction Pathway (106385); Downstream Signaling Events Of B Cell Receptor (BCR) Pathway (576250); Downstream Signaling Of Activated FGFR Pathway (160957); Endochondral Ossification Pathway (198812)

The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth, and invasion. It has been shown in vitro that this protein is able to induce neurite outgrowth in PC12 cells. Studies of the similar proteins in mouse and chick suggested that this protein is a pleiotropic growth factor that stimulates proliferation in a number of tissues, most notably the liver and small intestine. Knockout studies of the similar gene in mice implied the role of this protein in regulating proliferation and differentiation of midline cerebellar structures. [provided by RefSeq, Jul 2008]

FGF18: Plays an important role in the regulation of cell proliferation, cell differentiation and cell migration. Required for normal ossification and bone development. Stimulates hepatic and intestinal proliferation. Belongs to the heparin-binding growth factors family. Protein type: Cytokine; Secreted, signal peptide; Secreted. Chromosomal Location of Human Ortholog: 5q34. Cellular Component: extracellular space; extracellular region; nucleolus. Molecular Function: growth factor activity; type 2 fibroblast growth factor receptor binding; type 1 fibroblast growth factor receptor binding. Biological Process: epidermal growth factor receptor signaling pathway; nervous system development; intramembranous ossification; regulation of endothelial cell proliferation; anatomical structure morphogenesis; fibroblast growth factor receptor signaling pathway; phosphoinositide-mediated signaling; nerve growth factor receptor signaling pathway; chondrocyte development; signal transduction; positive regulation of vascular endothelial growth factor receptor signaling pathway; positive regulation of MAP kinase activity; positive regulation of chondrocyte differentiation; cell-cell signaling; positive regulation of cell proliferation; insulin receptor signaling pathway; innate immune response; angiogenesis; endochondral ossification; lung development

24-Strip-Wells

270 USD

48-Strip-Wells

505

96-Strip-Wells

685

5x96-Strip-Wells

2770

10x96-Strip-Wells

5025