ELISA/assay

Fatty Acid Binding Protein 3, Muscle And Heart (FABP3) ELISA Kit

MBS2020985

48-Strip-Wells

540 USD

ELISA Kit

Fatty Acid Binding Protein 3, Muscle And Heart (FABP3) ELISA Kit

Fatty Acid Binding Protein 3, Muscle And Heart (FABP3)

MDGI; FABP11; H-FABP; O-FABP; Hfabp; Mammary Derived Growth Inhibitor; Fatty Acid Binding Protein 11; Muscle fatty acid-binding protein; Heart-type fatty acid-binding protein

fatty acid binding protein 3, muscle and heart (mammary-derived growth inhibitor), isoform CRA_b; Fatty acid-binding protein, heart; fatty acid-binding protein, heart; fatty acid binding protein 3, muscle and heart; Fatty acid-binding protein 3; Heart-type fatty acid-binding protein; H-FABP; Mammary-derived growth inhibitor; MDGI; Muscle fatty acid-binding protein; M-FABP

FABP3

FABP3; FABP3; MDGI; FABP11; H-FABP; M-FABP; O-FABP; FABP11; MDGI; H-FABP; MDGI; M-FABP

FABPH_HUMAN

Human

133

This assay has high sensitivity and excellent specificity for detection of Fatty Acid Binding Protein 3, Muscle And Heart (FABP3). No significant cross-reactivity or interference between Fatty Acid Binding Protein 3, Muscle And Heart (FABP3) and analogues was observed.

For unopened kit: All the reagents should be kept according to the labels on vials. The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 degree C upon receipt while the others should be at 4 degree C. For opened kit: When the kit is opened, the remaining reagents still need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and reseal along entire edge of zip-seal. The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Samples: Serum, plasma, tissue homogenates, Cell lysates, cell culture supernates and other biological fluids. Assay Type: Sandwich. Detection Range: 0.625-40ng/mL. Sensitivity: Typically less than 0.273ng/mL.

Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Fatty Acid Binding Protein 3, Muscle And Heart (FABP3) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Fatty Acid Binding Protein 3, Muscle And Heart (FABP3) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 . Intra-Assay: CV<10% . Inter-Assay: CV<12% . Assay Procedure Summary: 1. Prepare all reagents, samples and standards;. 2. Add 100uL standard or sample to each well. Incubate 2 hours at 37 degree C;. 3. Aspirate and add 100uL prepared Detection Reagent A. Incubate 1 hour at 37 degree C;. 4. Aspirate and wash 3 times;. 5. Add 100uL prepared Detection Reagent B. Incubate 30 minutes at 37 degree C;. 6. Aspirate and wash 5 times;. 7. Add 90uL Substrate Solution. Incubate 15-25 minutes at 37 degree C;. 8. Add 50uL Stop Solution. Read at 450nm immediately.

Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Fatty Acid Binding Protein 3, Muscle And Heart (FABP3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Fatty Acid Binding Protein 3, Muscle And Heart (FABP3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Fatty Acid Binding Protein 3, Muscle And Heart (FABP3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Fatty Acid Binding Protein 3, Muscle And Heart (FABP3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

119628024

EAX07619.1

P05413

14,858 Da

PPAR Signaling Pathway (83042); PPAR Signaling Pathway (450)

The intracellular fatty acid-binding proteins (FABPs) belongs to a multigene family. FABPs are divided into at least three distinct types, namely the hepatic-, intestinal- and cardiac-type. They form 14-15 kDa proteins and are thought to participate in the uptake, intracellular metabolism and/or transport of long-chain fatty acids. They may also be responsible in the modulation of cell growth and proliferation. Fatty acid-binding protein 3 gene contains four exons and its function is to arrest growth of mammary epithelial cells. This gene is a candidate tumor suppressor gene for human breast cancer. [provided by RefSeq, Jul 2008]

FABP3: FABP are thought to play a role in the intracellular transport of long-chain fatty acids and their acyl-CoA esters. Belongs to the calycin superfamily. Fatty-acid binding protein (FABP) family. Protein type: Lipid-binding. Chromosomal Location of Human Ortholog: 1p33-p32. Cellular Component: extracellular space; sarcoplasm; cytosol. Molecular Function: protein binding; icosatetraenoic acid binding; cytoskeletal protein binding; long-chain fatty acid transporter activity. Biological Process: response to drug; negative regulation of cell proliferation; cholesterol homeostasis; regulation of fatty acid oxidation; fatty acid metabolic process; response to insulin stimulus; phospholipid homeostasis

48-Strip-Wells

540 USD

96-Strip-Wells

730

5x96-Strip-Wells

2830

10x96-Strip-Wells

5080