ELISA/assay

Mucin 5 Subtype AC (MUC5AC) ELISA Kit

MBS2020974

24-Strip-Wells

255 USD

ELISA Kit

Mucin 5 Subtype AC (MUC5AC) ELISA Kit

[Mucin 5 Subtype AC (MUC5AC)]

[TBM; MUC5; LeB; Oligomeric Mucus/Gel-Forming; Mucin 5,Subtypes A And C,Tracheobronchial/Gastric; Gastric mucin; Lewis B blood group antigen; Major airway glycoprotein]

[mucin-5AC; Mucin-5AC; mucin-5AC; mucin 5AC, oligomeric mucus/gel-forming; Gastric mucin]

[MUC5AC]

[MUC5AC; MUC5AC; TBM; leB; MUC5; mucin; TBM]

MUC5A_HUMAN

Human

5654

This assay has high sensitivity and excellent specificity for detection of MUC2. No significant cross-reactivity or interference between MUC2 and analogues was observed.

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Samples: Rat Tissue Homogenates, Cell Lysates And Other Biological Fluids. Assay Type: Quantitative Sandwich. Detection Range: 0.312-20ng/mL. Sensitivity: 0.113ng/mL.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level MUC2 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level MUC2 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%

Tumor immunity; Infection immunity; Endocrinology; Pulmonology

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of MUC2 in rat tissue homogenates, cell lysates and other biological fluids. Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to MUC2. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to MUC2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain MUC2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of MUC2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

748983076

NP_001291288.1

NM_001304359.1

585,570 Da

MUC5AC iso1: . Chromosomal Location of Human Ortholog: 11p15.5. Cellular Component: Golgi lumen; fibril. Molecular Function: extracellular matrix structural constituent. Biological Process: protein amino acid O-linked glycosylation; cellular protein metabolic process; O-glycan processing; fibril organization and biogenesis; post-translational protein modification

24-Strip-Wells

255 USD

48-Strip-Wells

455

96-Strip-Wells

610

5x96-Strip-Wells

2470

10x96-Strip-Wells

4445