ELISA/assay

Osteocalcin (OC) ELISA Kit

MBS2020904

48-Strip-Wells

525 USD

ELISA Kit

Osteocalcin (OC) ELISA Kit

Osteocalcin (OC)

BGLAP; OT; BGP; Bone Gla Protein; Bone Gamma-Carboxyglutamate Protein; Gamma-carboxyglutamic acid-containing protein

osteocalcin preproprotein; Osteocalcin; osteocalcin; bone gamma-carboxyglutamate (gla) protein; Bone Gla protein; BGP; Gamma-carboxyglutamic acid-containing protein

OC

BGLAP; BGLAP; OC; BGP; OCN; BGP

OSTCN_HUMAN

Mouse

100

This assay has high sensitivity and excellent specificity for detection of Osteocalcin (OC). No significant cross-reactivity or interference between Osteocalcin (OC) and analogues was observed.

For unopened kit: All the reagents should be kept according to the labels on vials. The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 degree C upon receipt while the others should be at 4 degree C. For opened kit: When the kit is opened, the remaining reagents still need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and reseal along entire edge of zip-seal. The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Samples: Serum, plasma, tissue homogenates and other biological fluids. Assay Type: Sandwich. Detection Range: 31.25-2000pg/mL. Sensitivity: Typically less than 12.7pg/mL.

Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Osteocalcin (OC) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Osteocalcin (OC) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 . Intra-Assay: CV<10% . Inter-Assay: CV<12% . Assay Procedure Summary: 1. Prepare all reagents, samples and standards;. 2. Add 100uL standard or sample to each well. Incubate 2 hours at 37 degree C;. 3. Aspirate and add 100uL prepared Detection Reagent A. Incubate 1 hour at 37 degree C;. 4. Aspirate and wash 3 times;. 5. Add 100uL prepared Detection Reagent B. Incubate 30 minutes at 37 degree C;. 6. Aspirate and wash 5 times;. 7. Add 90uL Substrate Solution. Incubate 15-25 minutes at 37 degree C;. 8. Add 50uL Stop Solution. Read at 450nm immediately.

Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Osteocalcin (OC). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Osteocalcin (OC). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Osteocalcin (OC), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Osteocalcin (OC) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

40316933

NP_954642.1

NM_199173.5

P02818

10,963 Da

FGF Signaling Pathway (137989); Glucocorticoid Receptor Regulatory Network Pathway (138014); Interleukin-11 Signaling Pathway (698753); Notch-mediated HES/HEY Network Pathway (169347); Regulation Of Retinoblastoma Protein Pathway (137916); Validated Transcriptional Targets Of AP1 Family Members Fra1 And Fra2 Pathway (169349)

This gene encodes a highly abundant bone protein secreted by osteoblasts that regulates bone remodeling and energy metabolism. The encoded protein contains a Gla (gamma carboxyglutamate) domain, which functions in binding to calcium and hydroxyapatite, the mineral component of bone. Serum osteocalcin levels may be negatively correlated with metabolic syndrome. Read-through transcription exists between this gene and the neighboring upstream gene, PMF1 (polyamine-modulated factor 1), but the encoded protein only shows sequence identity with the upstream gene product. [provided by RefSeq, Jun 2015]

osteocalcin: Constitutes 1-2% of the total bone protein. It binds strongly to apatite and calcium. Belongs to the osteocalcin/matrix Gla protein family. Protein type: Secreted, signal peptide; Secreted; Cell development/differentiation. Chromosomal Location of Human Ortholog: 1q22. Cellular Component: Golgi apparatus; extracellular space; rough endoplasmic reticulum; cytoplasm; dendrite; perikaryon. Molecular Function: structural constituent of bone; hydroxyapatite binding; calcium ion binding; structural molecule activity. Biological Process: response to drug; response to gravity; regulation of bone resorption; response to glucocorticoid stimulus; cell aging; response to testosterone stimulus; bone mineralization; osteoblast development; odontogenesis; osteoblast differentiation; response to ethanol; response to vitamin D; regulation of osteoclast differentiation; response to vitamin K; response to mechanical stimulus; response to zinc ion; response to estrogen stimulus; response to hydroxyisoflavone; response to activity; cell adhesion; skeletal development; regulation of bone mineralization

48-Strip-Wells

525 USD

96-Strip-Wells

715

5x96-Strip-Wells

2760

10x96-Strip-Wells

4985