ELISA/assay

E1A Binding Protein P300 (EP300) ELISA Kit

MBS2020903

48-Strip-Wells

575 USD

ELISA Kit

E1A Binding Protein P300 (EP300) ELISA Kit

E1A Binding Protein P300 (EP300)

KAT3B; p300; Histone Acetyltransferase p300

E1A binding protein p300; Histone acetyltransferase p300; histone acetyltransferase p300; E1A binding protein p300; E1A-associated protein p300

EP300

EP300; EP300; p300; KAT3B; RSTS2; P300; p300 HAT

EP300_HUMAN

Mouse

2414

This assay has high sensitivity and excellent specificity for detection of E1A Binding Protein P300 (EP300). No significant cross-reactivity or interference between E1A Binding Protein P300 (EP300) and analogues was observed.

For unopened kit: All the reagents should be kept according to the labels on vials. The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 degree C upon receipt while the others should be at 4 degree C. For opened kit: When the kit is opened, the remaining reagents still need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and reseal along entire edge of zip-seal. The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Samples: Tissue homogenates, Cell lysates and other biological fluids. Assay Type: Sandwich. Detection Range: 0.156-10ng/mL. Sensitivity: Typically less than 0.055ng/mL.

Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level E1A Binding Protein P300 (EP300) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level E1A Binding Protein P300 (EP300) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 . Intra-Assay: CV<10% . Inter-Assay: CV<12% . Assay Procedure Summary: 1. Prepare all reagents, samples and standards;. 2. Add 100uL standard or sample to each well. Incubate 2 hours at 37 degree C;. 3. Aspirate and add 100uL prepared Detection Reagent A. Incubate 1 hour at 37 degree C;. 4. Aspirate and wash 3 times;. 5. Add 100uL prepared Detection Reagent B. Incubate 30 minutes at 37 degree C;. 6. Aspirate and wash 5 times;. 7. Add 90uL Substrate Solution. Incubate 15-25 minutes at 37 degree C;. 8. Add 50uL Stop Solution. Read at 450nm immediately.

Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to E1A Binding Protein P300 (EP300). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to E1A Binding Protein P300 (EP300). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain E1A Binding Protein P300 (EP300), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of E1A Binding Protein P300 (EP300) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

119580812

EAW60408.1

Q09472

264,161 Da

ATF-2 Transcription Factor Network Pathway (138006); Adherens Junction Pathway (83070); Adherens Junction Pathway (481); Androgen Receptor Signaling Pathway (198806); Attenuation Phase Pathway (1127556); BMAL1:CLOCK,NPAS2 Activates Circadian Gene Expression Pathway (477138); C-MYB Transcription Factor Network Pathway (138073); Cell Cycle Pathway (530733); Cell Cycle, Mitotic Pathway (105765); Cell Cycle Pathway (198811)

This gene encodes the adenovirus E1A-associated cellular p300 transcriptional co-activator protein. It functions as histone acetyltransferase that regulates transcription via chromatin remodeling and is important in the processes of cell proliferation and differentiation. It mediates cAMP-gene regulation by binding specifically to phosphorylated CREB protein. This gene has also been identified as a co-activator of HIF1A (hypoxia-inducible factor 1 alpha), and thus plays a role in the stimulation of hypoxia-induced genes such as VEGF. Defects in this gene are a cause of Rubinstein-Taybi syndrome and may also play a role in epithelial cancer. [provided by RefSeq, Jul 2008]

p300: a histone acetyltransferase and transcriptional co-activator that regulates transcription via chromatin remodeling. Acetylates all four core histones in nucleosomes. Related to CPB (CREB-binding protein), and like CPB can stimulate transcription through activation of CREB. Specifically inhibited by the adenovirus oncoprotein E1A. A co-activator of HIF1A (hypoxia-inducible factor 1 alpha), and thus plays a role in the stimulation of hypoxia-induced genes such as VEGF. Mediates cAMP-gene regulation by binding specifically to phosphorylated CREB protein. Methylated at R580 and R604 in the KIX domain by CARM1, which blocks association with CREB, inhibits CREB signaling and activates the apoptotic response. Also methylated at R2142 by CARM1, which impairs interaction with NCoA2. Protein type: Acetyltransferase; Motility/polarity/chemotaxis; EC 2.3.1.48; Transcription, coactivator/corepressor; Nuclear receptor co-regulator. Chromosomal Location of Human Ortholog: 22q13.2. Cellular Component: nucleoplasm; transcription factor complex; cytoplasm; nucleus; histone acetyltransferase complex. Molecular Function: lysine N-acetyltransferase activity; transcription activator binding; zinc ion binding; chromatin DNA binding; p53 binding; transcription coactivator activity; beta-catenin binding; transcription factor binding; protein binding; histone acetyltransferase activity; DNA binding; androgen receptor binding; acetyltransferase activity; transferase activity, transferring acyl groups; chromatin binding; nuclear hormone receptor binding. Biological Process: circadian rhythm; establishment and/or maintenance of chromatin architecture; viral reproduction; positive regulation of protein binding; apoptosis; regulation of cell cycle; heart development; positive regulation of transcription of target genes involved in unfolded protein response; negative regulation of transcription from RNA polymerase II promoter; regulation of transcription, DNA-dependent; positive regulation of interferon type I production; G2/M transition of mitotic cell cycle; DNA damage response, signal transduction by p53 class mediator resulting in induction of apoptosis; nervous system development; somitogenesis; skeletal muscle development; Notch signaling pathway; internal peptidyl-lysine acetylation; protein stabilization; transcription, DNA-dependent; organ morphogenesis; protein amino acid acetylation; response to estrogen stimulus; response to hypoxia; innate immune response; positive regulation of transcription factor activity; positive regulation of transcription from RNA polymerase II promoter; mitotic cell cycle; internal protein amino acid acetylation; N-terminal peptidyl-lysine acetylation; lung development. Disease: Rubinstein-taybi Syndrome 2; Rubinstein-taybi Syndrome 1; Colorectal Cancer

48-Strip-Wells

575 USD

96-Strip-Wells

785

5x96-Strip-Wells

3055

10x96-Strip-Wells

5495