ELISA/assay

Wingless Type MMTV Integration Site Family, Member 5B (WNT5B) ELISA Kit

MBS2020878

48-Strip-Wells

610 USD

ELISA Kit

Wingless Type MMTV Integration Site Family, Member 5B (WNT5B) ELISA Kit

Wingless Type MMTV Integration Site Family, Member 5B (WNT5B)

Wingless-type MMTV integration site family, member 5B; Protein Wnt-5b; protein Wnt-5b; wingless-type MMTV integration site family, member 5B

WNT5B

WNT5B; WNT5B

WNT5B_HUMAN

Human

359

This assay has high sensitivity and excellent specificity for detection of Wingless Type MMTV Integration Site Family, Member 5B (WNT5B). No significant cross-reactivity or interference between Wingless Type MMTV Integration Site Family, Member 5B (WNT5B) and analogues was observed.

For unopened kit: All the reagents should be kept according to the labels on vials. The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 degree C upon receipt while the others should be at 4 degree C. For opened kit: When the kit is opened, the remaining reagents still need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and reseal along entire edge of zip-seal. The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Samples: Tissue homogenates, Cell lysates and other biological fluids. Assay Type: Sandwich. Detection Range: 1.56-100ng/mL. Sensitivity: Typically less than 0.55ng/mL.

Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Wingless Type MMTV Integration Site Family, Member 5B (WNT5B) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Wingless Type MMTV Integration Site Family, Member 5B (WNT5B) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 . Intra-Assay: CV<10% . Inter-Assay: CV<12% . Assay Procedure Summary: 1. Prepare all reagents, samples and standards;. 2. Add 100uL standard or sample to each well. Incubate 2 hours at 37 degree C;. 3. Aspirate and add 100uL prepared Detection Reagent A. Incubate 1 hour at 37 degree C;. 4. Aspirate and wash 3 times;. 5. Add 100uL prepared Detection Reagent B. Incubate 30 minutes at 37 degree C;. 6. Aspirate and wash 5 times;. 7. Add 90uL Substrate Solution. Incubate 15-25 minutes at 37 degree C;. 8. Add 50uL Stop Solution. Read at 450nm immediately.

Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Wingless Type MMTV Integration Site Family, Member 5B (WNT5B). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Wingless Type MMTV Integration Site Family, Member 5B (WNT5B). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Wingless Type MMTV Integration Site Family, Member 5B (WNT5B), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Wingless Type MMTV Integration Site Family, Member 5B (WNT5B) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

12804649

AAH01749.1

Q9H1J7

40,323 Da

Adipogenesis Pathway (198832); Basal Cell Carcinoma Pathway (83113); Basal Cell Carcinoma Pathway (525); DNA Damage Response (only ATM Dependent) Pathway (198827); Disease Pathway (530764); HTLV-I Infection Pathway (373901); HTLV-I Infection Pathway (373889); Hedgehog Signaling Pathway (83063); Hedgehog Signaling Pathway (474); Hippo Signaling Pathway (749777)

The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It encodes a protein which shows 94% and 80% amino acid identity to the mouse Wnt5b protein and the human WNT5A protein, respectively. Alternative splicing of this gene generates 2 transcript variants. [provided by RefSeq, Jul 2008]

WNT5B: Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule which affects the development of discrete regions of tissues. Is likely to signal over only few cell diameters. Interacts with PORCN. Belongs to the Wnt family. Protein type: Secreted, signal peptide; Secreted. Chromosomal Location of Human Ortholog: 12p13.3. Cellular Component: proteinaceous extracellular matrix; extracellular space; cell surface; Golgi lumen; endoplasmic reticulum lumen; plasma membrane; extracellular region. Molecular Function: frizzled binding; receptor binding. Biological Process: fat cell differentiation; neuron differentiation; Wnt receptor signaling pathway; cell fate commitment; wound healing; positive regulation of fat cell differentiation; chondrocyte differentiation; positive regulation of cell migration

48-Strip-Wells

610 USD

96-Strip-Wells

830

5x96-Strip-Wells

3265

10x96-Strip-Wells

5870