ELISA/assay

Transferrin Receptor (TFR) ELISA Kit

MBS2020872

48-Strip-Wells

540 USD

ELISA Kit

Transferrin Receptor (TFR) ELISA Kit

Transferrin Receptor (TFR)

CD71; TFR1; P90; TFRC; T9; Trfr; sTfR; Transferrin receptor protein 1, serum form

transferrin receptor; Transferrin receptor protein 1; transferrin receptor protein 1; transferrin receptor; T9; p90; CD_antigen: CD71

TFR

TFRC; TFRC; T9; TR; TFR; p90; CD71; TFR1; TRFR; TR; TfR; TfR1; Trfr; sTfR

TFR1_HUMAN

Human

760

This assay has high sensitivity and excellent specificity for detection of Transferrin Receptor (TFR). No significant cross-reactivity or interference between Transferrin Receptor (TFR) and analogues was observed.

For unopened kit: All the reagents should be kept according to the labels on vials. The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 degree C upon receipt while the others should be at 4 degree C. For opened kit: When the kit is opened, the remaining reagents still need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and reseal along entire edge of zip-seal. The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Samples: Serum, plasma, tissue homogenates, Cell lysates and other biological fluids. Assay Type: Sandwich. Detection Range: 0.313-20ng/mL. Sensitivity: Typically less than 0.144ng/mL.

Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Transferrin Receptor (TFR) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Transferrin Receptor (TFR) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 . Intra-Assay: CV<10% . Inter-Assay: CV<12% . Assay Procedure Summary: 1. Prepare all reagents, samples and standards;. 2. Add 100uL standard or sample to each well. Incubate 2 hours at 37 degree C;. 3. Aspirate and add 100uL prepared Detection Reagent A. Incubate 1 hour at 37 degree C;. 4. Aspirate and wash 3 times;. 5. Add 100uL prepared Detection Reagent B. Incubate 30 minutes at 37 degree C;. 6. Aspirate and wash 5 times;. 7. Add 90uL Substrate Solution. Incubate 15-25 minutes at 37 degree C;. 8. Add 50uL Stop Solution. Read at 450nm immediately.

Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Transferrin Receptor (TFR). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Transferrin Receptor (TFR). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Transferrin Receptor (TFR), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Transferrin Receptor (TFR) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

339516

AAA61153.1

P02786

84,871 Da

Clathrin Derived Vesicle Budding Pathway (119545); Endocytosis Pathway (102279); Endocytosis Pathway (102181); FOXA2 And FOXA3 Transcription Factor Networks Pathway (137911); Golgi Associated Vesicle Biogenesis Pathway (119546); HIF-1 Signaling Pathway (695200); HIF-1-alpha Transcription Factor Network Pathway (138045); Hematopoietic Cell Lineage Pathway (83078); Hematopoietic Cell Lineage Pathway (489); Iron Metabolism In Placenta Pathway (672461)

This gene encodes a cell surface receptor necessary for cellular iron uptake by the process of receptor-mediated endocytosis. This receptor is required for erythropoiesis and neurologic development. Multiple alternatively spliced variants have been identified. [provided by RefSeq, Sep 2015]

TfR: the transferrin receptor. Regulates the cellular uptake of iron occurs via receptor-mediated endocytosis of ligand-occupied receptors into specialized endosomes. Endosomal acidification leads to iron release. The apotransferrin-receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system. Protein type: Membrane protein, integral; Cell surface; Receptor, misc. Chromosomal Location of Human Ortholog: 3q29. Cellular Component: extracellular space; cell surface; intracellular membrane-bound organelle; integral to plasma membrane; cytoplasmic membrane-bound vesicle; extracellular region; coated pit; recycling endosome; membrane; perinuclear region of cytoplasm; plasma membrane; melanosome; endosome; vesicle; external side of plasma membrane. Molecular Function: identical protein binding; protein binding; transferrin receptor activity; double-stranded RNA binding. Biological Process: receptor-mediated endocytosis; viral reproduction; cellular iron ion homeostasis; transferrin transport; positive regulation of bone resorption; regulation of cell growth; osteoclast differentiation; transmembrane transport; regulation of cell proliferation

48-Strip-Wells

540 USD

96-Strip-Wells

730

5x96-Strip-Wells

2830

10x96-Strip-Wells

5080