Hypoxia Inducible Factor 2 Alpha (HIF2a) ELISA Kit | MyBioSource MBS2020870 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Hypoxia Inducible Factor 2 Alpha (HIF2a) ELISA Kit

catalog :

MBS2020870

quantity :

48-Strip-Wells

price :

605 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS2020870

products type :

ELISA Kit

products full name :

Hypoxia Inducible Factor 2 Alpha (HIF2a) ELISA Kit

products short name :

Hypoxia Inducible Factor 2 Alpha (HIF2a)

products name syn :

EPAS1; HLF; MOP2; PASD2; BHLHE73; Endothelial PAS Domain 1; HIF-1 Alpha-Like Factor; Basic-helix-loop-helix-PAS protein MOP2; Class E basic helix-loop-helix protein 73

other names :

hypoxia inducible factor 2 alpha; Endothelial PAS domain-containing protein 1; endothelial PAS domain-containing protein 1; endothelial PAS domain protein 1; Hypoxia-inducible factor 2-alpha; HIF-2-alpha; HIF2-alpha

products gene name :

HIF2a

other gene names :

Epas1; Epas1; HLF; Hif2a; Hif2a; EPAS-1; HIF-2-alpha; HIF2-alpha

uniprot entry name :

EPAS1_RAT

reactivity :

Rat

sequence length :

874

specificity :

This assay has high sensitivity and excellent specificity for detection of Hypoxia Inducible Factor 2 Alpha (HIF2a). No significant cross-reactivity or interference between Hypoxia Inducible Factor 2 Alpha (HIF2a) and analogues was observed.

storage stability :

For unopened kit: All the reagents should be kept according to the labels on vials. The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 degree C upon receipt while the others should be at 4 degree C. For opened kit: When the kit is opened, the remaining reagents still need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and reseal along entire edge of zip-seal. The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

other info1 :

Samples: Tissue homogenates, Cell lysates and other biological fluids. Assay Type: Sandwich. Detection Range: 78.13-5000pg/mL. Sensitivity: Typically less than 30pg/mL.

other info2 :

Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Hypoxia Inducible Factor 2 Alpha (HIF2a) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Hypoxia Inducible Factor 2 Alpha (HIF2a) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 . Intra-Assay: CV<10% . Inter-Assay: CV<12% . Assay Procedure Summary: 1. Prepare all reagents, samples and standards;. 2. Add 100uL standard or sample to each well. Incubate 2 hours at 37 degree C;. 3. Aspirate and add 100uL prepared Detection Reagent A. Incubate 1 hour at 37 degree C;. 4. Aspirate and wash 3 times;. 5. Add 100uL prepared Detection Reagent B. Incubate 30 minutes at 37 degree C;. 6. Aspirate and wash 5 times;. 7. Add 90uL Substrate Solution. Incubate 15-25 minutes at 37 degree C;. 8. Add 50uL Stop Solution. Read at 450nm immediately.

products description :

Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Hypoxia Inducible Factor 2 Alpha (HIF2a). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Hypoxia Inducible Factor 2 Alpha (HIF2a). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Hypoxia Inducible Factor 2 Alpha (HIF2a), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Hypoxia Inducible Factor 2 Alpha (HIF2a) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

ncbi gi num :

8953577

ncbi acc num :

CAB96612.1

uniprot acc num :

Q9JHS1

ncbi mol weight :

96,718 Da

ncbi pathways :

Adipogenesis Pathway (198479); Cellular Response To Hypoxia Pathway (1121333); Cellular Responses To Stress Pathway (1121332); Oxygen-dependent Asparagine Hydroxylation Of Hypoxia-inducible Factor Alpha Pathway (1121335); Oxygen-dependent Proline Hydroxylation Of Hypoxia-inducible Factor Alpha Pathway (1121336); Pathways In Cancer (83493); Regulation Of Hypoxia-inducible Factor (HIF) By Oxygen Pathway (1121334); Regulation Of Gene Expression By Hypoxia-inducible Factor Pathway (1121337); Renal Cell Carcinoma Pathway (83495); Renal Cell Carcinoma Pathway (519)

ncbi summary :

activates luciferase activity after transfection [RGD, Feb 2006]

size1 :

48-Strip-Wells

price1 :

605 USD

size2 :

96-Strip-Wells

price2 :

820

size3 :

5x96-Strip-Wells

price3 :

3220

size4 :

10x96-Strip-Wells

price4 :

5795

more info or order :

MyBioSource product webpage