Meprin A Alpha (MEP1a) ELISA Kit | MyBioSource MBS2020854 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Meprin A Alpha (MEP1a) ELISA Kit

catalog :

MBS2020854

quantity :

48-Strip-Wells

price :

540 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS2020854

products type :

ELISA Kit

products full name :

Meprin A Alpha (MEP1a) ELISA Kit

products short name :

Meprin A Alpha (MEP1a)

products name syn :

PPHA; PABA Peptide Hydrolase; Endopeptidase-2; N-benzoyl-L-tyrosyl-P-amino-Benzoic Acid Hydrolase Subunit Alpha; PABA Peptide Hydrolase

other names :

meprin A subunit alpha; Meprin A subunit alpha; meprin A subunit alpha; meprin A, alpha (PABA peptide hydrolase); Endopeptidase-2; N-benzoyl-L-tyrosyl-P-amino-benzoic acid hydrolase subunit alpha; PABA peptide hydrolase; PPH alpha

products gene name :

MEP1a

other gene names :

MEP1A; MEP1A; PPHA

uniprot entry name :

MEP1A_HUMAN

reactivity :

Mouse

sequence length :

746

specificity :

This assay has high sensitivity and excellent specificity for detection of Meprin A Alpha (MEP1a). No significant cross-reactivity or interference between Meprin A Alpha (MEP1a) and analogues was observed.

storage stability :

For unopened kit: All the reagents should be kept according to the labels on vials. The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 degree C upon receipt while the others should be at 4 degree C. For opened kit: When the kit is opened, the remaining reagents still need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and reseal along entire edge of zip-seal. The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

other info1 :

Samples: Serum, plasma, tissue homogenates and other biological fluids. Assay Type: Sandwich. Detection Range: 62.5-4000pg/mL. Sensitivity: Typically less than 26.4pg/mL.

other info2 :

Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Meprin A Alpha (MEP1a) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Meprin A Alpha (MEP1a) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 . Intra-Assay: CV<10% . Inter-Assay: CV<12% . Assay Procedure Summary: 1. Prepare all reagents, samples and standards;. 2. Add 100uL standard or sample to each well. Incubate 2 hours at 37 degree C;. 3. Aspirate and add 100uL prepared Detection Reagent A. Incubate 1 hour at 37 degree C;. 4. Aspirate and wash 3 times;. 5. Add 100uL prepared Detection Reagent B. Incubate 30 minutes at 37 degree C;. 6. Aspirate and wash 5 times;. 7. Add 90uL Substrate Solution. Incubate 15-25 minutes at 37 degree C;. 8. Add 50uL Stop Solution. Read at 450nm immediately.

products description :

Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Meprin A Alpha (MEP1a). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Meprin A Alpha (MEP1a). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Meprin A Alpha (MEP1a), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Meprin A Alpha (MEP1a) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

ncbi gi num :

153070262

ncbi acc num :

NP_005579.2

ncbi gb acc num :

NM_005588.2

uniprot acc num :

Q16819

ncbi mol weight :

84,419 Da

ncbi pathways :

Protein Digestion And Absorption Pathway (172847); Protein Digestion And Absorption Pathway (171868)

uniprot summary :

MEP1A: Belongs to the peptidase M12A family. Protein type: Membrane protein, integral; Protease; EC 3.4.24.18. Chromosomal Location of Human Ortholog: 6p12-p11. Cellular Component: extracellular space; integral to plasma membrane; meprin A complex. Molecular Function: protein binding; zinc ion binding; metalloendopeptidase activity. Biological Process: digestion; proteolysis

size1 :

48-Strip-Wells

price1 :

540 USD

size2 :

96-Strip-Wells

price2 :

730

size3 :

5x96-Strip-Wells

price3 :

2760

size4 :

10x96-Strip-Wells

price4 :

4985

more info or order :

MyBioSource product webpage