ELISA/assay

A Disintegrin And Metalloproteinase With Thrombospondin 7 (ADAMTS7) ELISA Kit

MBS2020845

48-Strip-Wells

540 USD

ELISA Kit

A Disintegrin And Metalloproteinase With Thrombospondin 7 (ADAMTS7) ELISA Kit

A Disintegrin And Metalloproteinase With Thrombospondin 7 (ADAMTS7)

COMPase

A disintegrin and metalloproteinase with thrombospondin motifs 7 preproprotein; A disintegrin and metalloproteinase with thrombospondin motifs 7; A disintegrin and metalloproteinase with thrombospondin motifs 7; ADAM metallopeptidase with thrombospondin type 1 motif, 7; COMPase

ADAMTS7

ADAMTS7; ADAMTS7; ADAM-TS7; ADAMTS-7; ADAM-TS 7; ADAM-TS 7; ADAM-TS7; ADAMTS-7

ATS7_HUMAN

Human

1686

This assay has high sensitivity and excellent specificity for detection of A Disintegrin And Metalloproteinase With Thrombospondin 7 (ADAMTS7). No significant cross-reactivity or interference between A Disintegrin And Metalloproteinase With Thrombospondin 7 (ADAMTS7) and analogues was observed.

For unopened kit: All the reagents should be kept according to the labels on vials. The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 degree C upon receipt while the others should be at 4 degree C. For opened kit: When the kit is opened, the remaining reagents still need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and reseal along entire edge of zip-seal. The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Samples: Tissue homogenates, Cell lysates and other biological fluids. Assay Type: Sandwich. Detection Range: 0.156-10ng/mL. Sensitivity: Typically less than 0.057ng/mL.

Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level A Disintegrin And Metalloproteinase With Thrombospondin 7 (ADAMTS7) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level A Disintegrin And Metalloproteinase With Thrombospondin 7 (ADAMTS7) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 . Intra-Assay: CV<10% . Inter-Assay: CV<12% . Assay Procedure Summary: 1. Prepare all reagents, samples and standards;. 2. Add 100uL standard or sample to each well. Incubate 2 hours at 37 degree C;. 3. Aspirate and add 100uL prepared Detection Reagent A. Incubate 1 hour at 37 degree C;. 4. Aspirate and wash 3 times;. 5. Add 100uL prepared Detection Reagent B. Incubate 30 minutes at 37 degree C;. 6. Aspirate and wash 5 times;. 7. Add 90uL Substrate Solution. Incubate 15-25 minutes at 37 degree C;. 8. Add 50uL Stop Solution. Read at 450nm immediately.

Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to A Disintegrin And Metalloproteinase With Thrombospondin 7 (ADAMTS7). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to A Disintegrin And Metalloproteinase With Thrombospondin 7 (ADAMTS7). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain A Disintegrin And Metalloproteinase With Thrombospondin 7 (ADAMTS7), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of A Disintegrin And Metalloproteinase With Thrombospondin 7 (ADAMTS7) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

38683827

NP_055087.2

NM_014272.3

Q9UKP4

184,095 Da

Metabolism Of Proteins Pathway (106230); O-glycosylation Of TSR Domain-containing Proteins Pathway (1127680); O-linked Glycosylation Pathway (1127679); Post-translational Protein Modification Pathway (161012)

The protein encoded by this gene is a member of the ADAMTS (a disintegrin and metalloproteinase with thrombospondin motifs) family. Members of this family share several distinct protein modules, including a propeptide region, a metalloproteinase domain, a disintegrin-like domain, and a thrombospondin type 1 (TS) motif. Individual members of this family differ in the number of C-terminal TS motifs, and some have unique C-terminal domains. The protein encoded by this gene contains two C-terminal TS motifs. [provided by RefSeq, Jul 2008]

ADAMTS7: Metalloprotease that may play a role in the degradation of COMP. Protein type: Protease; Cell surface; Secreted, signal peptide; Motility/polarity/chemotaxis; Secreted; EC 3.4.24.-. Chromosomal Location of Human Ortholog: 15q24.2. Cellular Component: proteinaceous extracellular matrix; cell surface; endoplasmic reticulum lumen. Molecular Function: protein binding; metallopeptidase activity; zinc ion binding; metalloendopeptidase activity. Biological Process: protein amino acid O-linked glycosylation; cellular protein metabolic process; proteolysis involved in cellular protein catabolic process; negative regulation of chondrocyte differentiation; post-translational protein modification

48-Strip-Wells

540 USD

96-Strip-Wells

730

5x96-Strip-Wells

2830

10x96-Strip-Wells

5080