ELISA/assay

Heat Shock Protein 70 (HSP70) ELISA Kit

MBS2020836

24-Strip-Wells

255 USD

ELISA Kit

Heat Shock Protein 70 (HSP70) ELISA Kit

[Heat Shock Protein 70 (HSP70)]

[DnaK; 70 Kilodalton Heat Shock Proteins]

[heat shock 70 kDa protein; Heat shock 70 kDa protein; heat shock 70 kDa protein; heat shock 70kDa protein 2]

[HSP70]

[HSPA2; HSP70; HSP70; HSP70]

HSP70_CHICK

Human

634

This assay has high sensitivity and excellent specificity for detection of HSP70. No significant cross-reactivity or interference between HSP70 and analogues was observed.

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Samples: Gallus Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates And Other Biological Fluids. Assay Type: Quantitative Sandwich. Detection Range: 1.56-100ng/mL. Sensitivity: 0.59ng/mL.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level HSP70 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level HSP70 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%

Tumor immunity; Infection immunity; Developmental science

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of HSP70 in gallus serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids. Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to HSP70. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to HSP70. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain HSP70, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of HSP70 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

55742654

NP_001006686.1

NM_001006685.1

P08106

69,751 Da

Endocytosis Pathway (102290); Endocytosis Pathway (102181); Influenza A Pathway (217175); Influenza A Pathway (217150); MAPK Signaling Pathway (83956); MAPK Signaling Pathway (456); Protein Processing In Endoplasmic Reticulum Pathway (167335); Protein Processing In Endoplasmic Reticulum Pathway (167190); Spliceosome Pathway (125151); Spliceosome Pathway (124832)

24-Strip-Wells

255 USD

48-Strip-Wells

455

96-Strip-Wells

610

5x96-Strip-Wells

2470

10x96-Strip-Wells

4445