ELISA/assay

Choline Acetyltransferase (ChAT) ELISA Kit

MBS2020762

48-Strip-Wells

540 USD

ELISA Kit

Choline Acetyltransferase (ChAT) ELISA Kit

Choline Acetyltransferase (ChAT)

CMS1A; CMS1A2; CHOACTase; Choline acetylase

choline acetyltransferase, partial; Choline O-acetyltransferase; choline O-acetyltransferase; choline O-acetyltransferase

ChAT

CHAT; CHAT; CMS6; CMS1A; CMS1A2; CHOACTASE; CHOACTase; ChAT; Choline acetylase

CLAT_HUMAN

Human

95

This assay has high sensitivity and excellent specificity for detection of ChAT. No significant cross-reactivity or interference between ChAT and analogues was observed.

For unopened kit: All the reagents should be kept according to the labels on vials. The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 degree C upon receipt while the others should be at 4 degree C. For opened kit: When the kit is opened, the remaining reagents still need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and reseal along entire edge of zip-seal. The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Samples: Human tissue homogenates, cell lysates and other biological fluids. Assay Type: Sandwich. Detection Range: 0.156-10ng/mL. The standard curve concentrations used for the ELISA's were 10ng/mL, 5ng/mL, 2.5ng/mL, 1.25ng/mL, 0.625ng/mL, 0.312ng/mL, 0.156ng/mL. Sensitivity: 0.059ng/mL.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level ChAT were tested 20 times on one plate, respectively. Intra-Assay: CV<10%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level ChAT were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of ChAT in human tissue homogenates, cell lysates and other biological fluids. Principle of the Assay The microtiter plate provided in this kit has been pre-coated with an antibody specific to ChAT. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to ChAT. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain ChAT, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of ChAT in the samples is then determined by comparing the O.D. of the samples to the standard curve.

553229

AAC37590.1

P28329

70,394 Da

Acetylcholine Neurotransmitter Release Cycle Pathway (106525); Acetylcholine Synthesis Pathway (198820); Biogenic Amine Synthesis Pathway (198793); Cholinergic Synapse Pathway (217716); Glycerophospholipid Biosynthesis Pathway (645313); Glycerophospholipid Metabolism Pathway (82989); Glycerophospholipid Metabolism Pathway (364); Metabolism Pathway (477135); Metabolism Of Lipids And Lipoproteins Pathway (160976); Neuronal System Pathway (106513)

This gene encodes an enzyme which catalyzes the biosynthesis of the neurotransmitter acetylcholine. This gene product is a characteristic feature of cholinergic neurons, and changes in these neurons may explain some of the symptoms of Alzheimer's disease. Polymorphisms in this gene have been associated with Alzheimer's disease and mild cognitive impairment. Mutations in this gene are associated with congenital myasthenic syndrome associated with episodic apnea. Multiple transcript variants encoding different isoforms have been found for this gene, and some of these variants have been shown to encode more than one isoform. [provided by RefSeq, May 2010]

CHAT: an enzyme that catalyzes the reversible synthesis of acetylcholine (ACh) from acetyl CoA and choline at cholinergic synapses. Four alternative splice variants have been described. Protein type: EC 2.3.1.6; Acetyltransferase; Lipid Metabolism - glycerophospholipid. Chromosomal Location of Human Ortholog: 10q11.2. Cellular Component: cell soma; mitochondrion; axon; cytoplasm; nucleus; cytosol. Molecular Function: choline O-acetyltransferase activity. Biological Process: synaptic transmission; muscle development; rhythmic excitation; rhythmic behavior; phospholipid metabolic process; neurotransmitter secretion; glycerophospholipid biosynthetic process; phosphatidylcholine biosynthetic process; dendrite development; neuromuscular synaptic transmission; establishment of synaptic specificity at neuromuscular junction; adult walking behavior; neurotransmitter biosynthetic process. Disease: Myasthenic Syndrome, Congenital, Associated With Episodic Apnea

48-Strip-Wells

540 USD

96-Strip-Wells

730

5x96-Strip-Wells

2830

10x96-Strip-Wells

5080