catalog number :
MBS2020709
products type :
ELISA Kit
products full name :
Lymphocyte Antigen 9 (LY9) ELISA Kit
products short name :
Lymphocyte Antigen 9 (LY9)
products name syn :
CD229; LY9; SLAMF3; Hly9; Mly9; SLAMF3; Cell surface molecule Ly-9; Signaling lymphocytic activation molecule 3
other names :
Lymphocyte antigen 9; T-lymphocyte surface antigen Ly-9; T-lymphocyte surface antigen Ly-9; lymphocyte antigen 9; Cell surface molecule Ly-9; Lymphocyte antigen 9; SLAM family member 3; SLAMF3; Signaling lymphocytic activation molecule 3; CD_antigen: CD229
other gene names :
LY9; LY9; hly9; mLY9; CD229; SLAMF3; SLAMF3
uniprot entry name :
LY9_HUMAN
specificity :
This assay has high sensitivity and excellent specificity for detection of Lymphocyte Antigen 9 (LY9). No significant cross-reactivity or interference between Lymphocyte Antigen 9 (LY9) and analogues was observed.
storage stability :
For unopened kit: All the reagents should be kept according to the labels on vials. The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 degree C upon receipt while the others should be at 4 degree C. For opened kit: When the kit is opened, the remaining reagents still need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and reseal along entire edge of zip-seal. The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
other info1 :
Samples: Tissue homogenates, Cell lysates and other biological fluids. Assay Type: Sandwich. Detection Range: 0.313-20ng/mL. Sensitivity: Typically less than 0.128ng/mL.
other info2 :
Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Lymphocyte Antigen 9 (LY9) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Lymphocyte Antigen 9 (LY9) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 . Intra-Assay: CV<10% . Inter-Assay: CV<12% . Assay Procedure Summary: 1. Prepare all reagents, samples and standards;. 2. Add 100uL standard or sample to each well. Incubate 2 hours at 37 degree C;. 3. Aspirate and add 100uL prepared Detection Reagent A. Incubate 1 hour at 37 degree C;. 4. Aspirate and wash 3 times;. 5. Add 100uL prepared Detection Reagent B. Incubate 30 minutes at 37 degree C;. 6. Aspirate and wash 5 times;. 7. Add 90uL Substrate Solution. Incubate 15-25 minutes at 37 degree C;. 8. Add 50uL Stop Solution. Read at 450nm immediately.
products description :
Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Lymphocyte Antigen 9 (LY9). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Lymphocyte Antigen 9 (LY9). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Lymphocyte Antigen 9 (LY9), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Lymphocyte Antigen 9 (LY9) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ncbi acc num :
AAH64485.1
ncbi mol weight :
71,961 Da
ncbi summary :
LY9 belongs to the SLAM family of immunomodulatory receptors (see SLAMF1; MIM 603492) and interacts with the adaptor molecule SAP (SH2D1A; MIM 300490) (Graham et al., 2006 [PubMed 16365421]).[supplied by OMIM, Mar 2008]
uniprot summary :
CD229: May participate in adhesion reactions between T lymphocytes and accessory cells by homophilic interaction. 4 isoforms of the human protein are produced by alternative splicing. Protein type: Cell surface; Membrane protein, integral. Chromosomal Location of Human Ortholog: 1q23.3. Cellular Component: integral to membrane. Molecular Function: receptor activity. Biological Process: cell adhesion
size4 :
10x96-Strip-Wells