Trefoil Factor 3, Intestinal (TFF3) ELISA Kit | MyBioSource MBS2020687 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Trefoil Factor 3, Intestinal (TFF3) ELISA Kit

catalog :

MBS2020687

quantity :

48-Strip-Wells

price :

575 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS2020687

products type :

ELISA Kit

products full name :

Trefoil Factor 3, Intestinal (TFF3) ELISA Kit

products short name :

Trefoil Factor 3, Intestinal (TFF3)

products name syn :

HITF; ITF3; TFI; HP1.B

other names :

trefoil factor 3; Trefoil factor 3; trefoil factor 3; trefoil factor 3 (intestinal); Intestinal trefoil factor; hITF; Polypeptide P1.B; hP1.B

products gene name :

TFF3

other gene names :

TFF3; TFF3; ITF; P1B; TFI; ITF; TFI; hITF; hP1.B

uniprot entry name :

TFF3_HUMAN

reactivity :

Rat

sequence length :

specificity :

This assay has high sensitivity and excellent specificity for detection of Trefoil Factor 3, Intestinal (TFF3). No significant cross-reactivity or interference between Trefoil Factor 3, Intestinal (TFF3) and analogues was observed.

storage stability :

For unopened kit: All the reagents should be kept according to the labels on vials. The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 degree C upon receipt while the others should be at 4 degree C. For opened kit: When the kit is opened, the remaining reagents still need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and reseal along entire edge of zip-seal. The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

other info1 :

Samples: Serum, plasma, tissue homogenates and other biological fluids. Assay Type: Sandwich. Detection Range: 46.88-3000pg/mL. Sensitivity: Typically less than 19.54pg/mL.

other info2 :

Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Trefoil Factor 3, Intestinal (TFF3) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Trefoil Factor 3, Intestinal (TFF3) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 . Intra-Assay: CV<10% . Inter-Assay: CV<12% . Assay Procedure Summary: 1. Prepare all reagents, samples and standards;. 2. Add 100uL standard or sample to each well. Incubate 2 hours at 37 degree C;. 3. Aspirate and add 100uL prepared Detection Reagent A. Incubate 1 hour at 37 degree C;. 4. Aspirate and wash 3 times;. 5. Add 100uL prepared Detection Reagent B. Incubate 30 minutes at 37 degree C;. 6. Aspirate and wash 5 times;. 7. Add 90uL Substrate Solution. Incubate 15-25 minutes at 37 degree C;. 8. Add 50uL Stop Solution. Read at 450nm immediately.

products description :

Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Trefoil Factor 3, Intestinal (TFF3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Trefoil Factor 3, Intestinal (TFF3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Trefoil Factor 3, Intestinal (TFF3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Trefoil Factor 3, Intestinal (TFF3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

ncbi gi num :

281485608

ncbi acc num :

NP_003217.3

ncbi gb acc num :

NM_003226.3

uniprot acc num :

Q07654

ncbi mol weight :

8,641 Da

ncbi pathways :

HIF-1-alpha Transcription Factor Network Pathway (138045); IL4-mediated Signaling Events Pathway (137933)

ncbi summary :

Members of the trefoil family are characterized by having at least one copy of the trefoil motif, a 40-amino acid domain that contains three conserved disulfides. They are stable secretory proteins expressed in gastrointestinal mucosa. Their functions are not defined, but they may protect the mucosa from insults, stabilize the mucus layer and affect healing of the epithelium. This gene is expressed in goblet cells of the intestines and colon. This gene and two other related trefoil family member genes are found in a cluster on chromosome 21. [provided by RefSeq, Jul 2008]

uniprot summary :

TFF3: Involved in the maintenance and repair of the intestinal mucosa. Promotes the mobility of epithelial cells in healing processes (motogen). Protein type: Secreted, signal peptide; Secreted. Chromosomal Location of Human Ortholog: 21q22.3. Cellular Component: proteinaceous extracellular matrix; extracellular region; secretory granule. Biological Process: response to peptide hormone stimulus; digestion; defense response

size1 :

48-Strip-Wells

price1 :

575 USD

size2 :

96-Strip-Wells

price2 :

785

size3 :

5x96-Strip-Wells

price3 :

3065

size4 :

10x96-Strip-Wells

price4 :

5510

more info or order :

MyBioSource product webpage