ELISA/assay

Wingless Type MMTV Integration Site Family, Member 7B (WNT7B) ELISA Kit

MBS2020668

48-Strip-Wells

610 USD

ELISA Kit

Wingless Type MMTV Integration Site Family, Member 7B (WNT7B) ELISA Kit

Wingless Type MMTV Integration Site Family, Member 7B (WNT7B)

wingless-type MMTV integration site family, member 7B; Protein Wnt-7b; protein Wnt-7b; wingless-type MMTV integration site family, member 7B

WNT7B

WNT7B; WNT7B

WNT7B_HUMAN

Human

177

This assay has high sensitivity and excellent specificity for detection of WNT7B. No significant cross-reactivity or interference between WNT7B and analogues was observed.

For unopened kit: All the reagents should be kept according to the labels on vials. The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 degree C upon receipt while the others should be at 4 degree C. For opened kit: When the kit is opened, the remaining reagents still need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and reseal along entire edge of zip-seal. The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Samples: Human tissue homogenates, cell lysates and other biological fluids. Assay Type: Sandwich. Detection Range: 0.156-10ng/mL. The standard curve concentrations used for the ELISA's were 10ng/mL, 5ng/mL, 2.5ng/mL, 1.25ng/mL, 0.625ng/mL, 0.312ng/mL, 0.156ng/mL. Sensitivity: Typically less than 0.053ng/mL

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level WNT7B were tested 20 times on one plate, respectively. Intra-Assay: CV<10%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level WNT7B were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%

Intended Uses The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of WNT7B in human tissue homogenates, cell lysates and other biological fluids. Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to WNT7B. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to WNT7B. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain WNT7B, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of WNT7B in the samples is then determined by comparing the O.D. of the samples to the standard curve.

119593800

EAW73394.1

P56706

39,327 Da

Basal Cell Carcinoma Pathway (83113); Basal Cell Carcinoma Pathway (525); Class B/2 (Secretin Family Receptors) Pathway (106378); DNA Damage Response (only ATM Dependent) Pathway (198827); Defective ACTH Causes Obesity And Pro-opiomelanocortinin Deficiency (POMCD) Pathway (1127664); Disease Pathway (530764); GPCR Ligand Binding Pathway (161020); HTLV-I Infection Pathway (373901); HTLV-I Infection Pathway (373889); Hedgehog Signaling Pathway (83063)

This gene is a member of the WNT gene family, which consists of structurally related genes that encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. Among members of the human WNT family, this gene product is most similar to WNT7A protein. [provided by RefSeq, Oct 2008]

WNT7B: Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule which affects the development of discrete regions of tissues. Is likely to signal over only few cell diameters. Belongs to the Wnt family. Protein type: Secreted, signal peptide; Secreted. Chromosomal Location of Human Ortholog: 22q13. Cellular Component: proteinaceous extracellular matrix; extracellular space; endoplasmic reticulum lumen; Golgi lumen; plasma membrane; extracellular region. Molecular Function: frizzled binding. Biological Process: oxygen homeostasis; cell fate commitment; Wnt receptor signaling pathway; in utero embryonic development; response to glucocorticoid stimulus; positive regulation of JNK cascade; Wnt receptor signaling pathway through beta-catenin; forebrain regionalization; anatomical structure regression; activation of JNK activity; odontogenesis of dentine-containing teeth; neuron differentiation; positive regulation of osteoblast differentiation; cellular metabolic process; central nervous system vasculogenesis; fibroblast proliferation; homeostatic process; negative regulation of smoothened signaling pathway; positive regulation of cell proliferation; regulation of cell projection size; synapse organization and biogenesis; embryonic organ development; smooth muscle cell differentiation; establishment and/or maintenance of polarity of embryonic epithelium; neurite morphogenesis; lung development

48-Strip-Wells

610 USD

96-Strip-Wells

830

5x96-Strip-Wells

3265

10x96-Strip-Wells

5870