ELISA/assay

Matrix Metalloproteinase 8 (MMP8) ELISA Kit

MBS2020582

48-Strip-Wells

325 USD

ELISA Kit

Matrix Metalloproteinase 8 (MMP8) ELISA Kit

Matrix Metalloproteinase 8 (MMP8)

CLG2; HNC; PMNL-CL; Collagenase 2; Neutrophil Collagenase; PMNL collagenase

matrix metalloproteinase 8 (neutrophil collagenase); Neutrophil collagenase; neutrophil collagenase; matrix metallopeptidase 8; Matrix metalloproteinase-8; MMP-8; PMNL collagenase; PMNL-CL

MMP8

MMP8; MMP8; HNC; CLG1; MMP-8; PMNL-CL; CLG1; MMP-8; PMNL-CL

MMP8_HUMAN

Human

467

This assay has high sensitivity and excellent specificity for detection of Matrix Metalloproteinase 8 (MMP8). No significant cross-reactivity or interference between Matrix Metalloproteinase 8 (MMP8) and analogues was observed.

For unopened kit: All the reagents should be kept according to the labels on vials. The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 degree C upon receipt while the others should be at 4 degree C. For opened kit: When the kit is opened, the remaining reagents still need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and reseal along entire edge of zip-seal. The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Samples: Serum, plasma, tissue homogenates, Cell lysates, cell culture supernates and other biological fluids. Assay Type: Sandwich. Detection Range: 78.13-5000pg/mL. Sensitivity: Typically less than 31pg/mL.

Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Matrix Metalloproteinase 8 (MMP8) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Matrix Metalloproteinase 8 (MMP8) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 . Intra-Assay: CV<10% . Inter-Assay: CV<12% . Assay Procedure Summary: 1. Prepare all reagents, samples and standards;. 2. Add 100uL standard or sample to each well. Incubate 2 hours at 37 degree C;. 3. Aspirate and add 100uL prepared Detection Reagent A. Incubate 1 hour at 37 degree C;. 4. Aspirate and wash 3 times;. 5. Add 100uL prepared Detection Reagent B. Incubate 30 minutes at 37 degree C;. 6. Aspirate and wash 5 times;. 7. Add 90uL Substrate Solution. Incubate 15-25 minutes at 37 degree C;. 8. Add 50uL Stop Solution. Read at 450nm immediately.

Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Matrix Metalloproteinase 8 (MMP8). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Matrix Metalloproteinase 8 (MMP8). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Matrix Metalloproteinase 8 (MMP8), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Matrix Metalloproteinase 8 (MMP8) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

71648770

AAZ38714.1

P22894

53,412 Da

Activation Of Matrix Metalloproteinases Pathway (576264); Collagen Degradation Pathway (730309); Degradation Of The Extracellular Matrix Pathway (576263); Extracellular Matrix Organization Pathway (576262); Matrix Metalloproteinases Pathway (198900)

This gene encodes a member of the matrix metalloproteinase (MMP) family of proteins. These proteins are involved in the breakdown of extracellular matrix in embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Proteolysis at different sites on this protein results in multiple active forms of the enzyme with distinct N-termini. This protein functions in the degradation of type I, II and III collagens. The gene is part of a cluster of MMP genes which localize to chromosome 11q22.3. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2015]

MMP8: Can degrade fibrillar type I, II, and III collagens. Belongs to the peptidase M10A family. Protein type: Secreted; Secreted, signal peptide; Protease; EC 3.4.24.34. Chromosomal Location of Human Ortholog: 11q22.3. Cellular Component: proteinaceous extracellular matrix; extracellular space; extracellular region. Molecular Function: zinc ion binding; serine-type endopeptidase activity; metalloendopeptidase activity; calcium ion binding. Biological Process: extracellular matrix disassembly; collagen catabolic process; extracellular matrix organization and biogenesis; proteolysis

48-Strip-Wells

325 USD

96-Strip-Wells

425

5x96-Strip-Wells

1530

10x96-Strip-Wells

2715