This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
MyBioSource
product type :
ELISA/assay
product name :
Indoleamine-2,3-Dioxygenase (IDO) ELISA Kit
catalog :
MBS2020539
quantity :
24-Strip-Wells
price :
265 USD
product information
catalog number :
MBS2020539
products type :
ELISA Kit
products full name :
Indoleamine-2,3-Dioxygenase (IDO) ELISA Kit
products short name :
[Indoleamine-2,3-Dioxygenase (IDO)]
products name syn :
[CD107B; INDO; Indoleamine-Pyrrole 2,3 Dioxygenase]
other names :
[Indoleamine 2,3-dioxygenase; Indoleamine 2,3-dioxygenase; dioxygenase BNA2; Biosynthesis of nicotinic acid protein 2]
products gene name :
[IDO]
other gene names :
[BNA2; BNA2; IDO]
uniprot entry name :
I23O_YEAST
reactivity :
Mouse
sequence length :
453
specificity :
This assay has high sensitivity and excellent specificity for detection of Indoleamine-2,3-Dioxygenase (IDO). No significant cross-reactivity or interference between Indoleamine-2,3-Dioxygenase (IDO) and analogues was observed.
storage stability :
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
image1 heading :
Typical Testing Data/Standard Curve (for reference only)
other info1 :
Assay Type: Double-antibody Sandwich. Samples: Serum, Plasma, Tissue homogenates and Other Biological Fluids. Detection Range: 15.6-1,000pg/mL. Sensitivity: < 6.1pg/mL
other info2 :
Application: Enzyme-linked immunosorbent assay for Antigen Detection. Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Indoleamine-2,3-Dioxygenase (IDO) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Indoleamine-2,3-Dioxygenase (IDO) were tested on 3 different plates, 8 replicates in each plate. CV(%) =: SD/meanX100. Intra-Assay: CV<10%. Inter-Assay: CV<12%
products categories :
Enzyme Infection immunity
products description :
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Indoleamine-2,3-Dioxygenase (IDO). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Indoleamine-2,3-Dioxygenase (IDO). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Indoleamine-2,3-Dioxygenase (IDO), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Indoleamine-2,3-Dioxygenase (IDO) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ncbi gi num :
1176339
ncbi acc num :
P47125.1
uniprot acc num :
P47125
ncbi mol weight :
50,775 Da
ncbi pathways :
De Novo NAD Biosynthesis Pathway (198675); Metabolic Pathways (131993); Metabolism Pathway (1122041); Metabolism Of Amino Acids And Derivatives Pathway (1122175); NAD Biosynthesis II (from Tryptophan) Pathway (143526); NAD Biosynthesis II (from Tryptophan) Pathway (138368); Tryptophan Degradation Via Kynurenine Pathway (198749); Tryptophan Catabolism Pathway (1122182); Tryptophan Metabolism Pathway (889); Tryptophan Metabolism Pathway (332)
uniprot summary :
Catalyzes the first step in tryptophan catabolism in order to supply de novo nicotinamide adenine dinucleotide (NAD+) via the kynurenine pathway. Plays a role in the cellular response to telomere uncapping.
size1 :
24-Strip-Wells
price1 :
265 USD
size2 :
48-Strip-Wells
price2 :
490
size3 :
96-Strip-Wells
price3 :
655
size4 :
5x96-Strip-Wells
price4 :
2660
size5 :
10x96-Strip-Wells
price5 :
4795
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com
1-888-627-0165
headquarters: USA
MyBioSource, LLC was orginally founded in Vancouver by three enthusiastic scientists who are passionate about providing the world with the best reagents available. Together, they form a company with a big vision known as MyBioSource. MyBioSource is now located in San Diego, California, USA.

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