ELISA/assay

Inducible T-Cell Co Stimulator (ICOS) ELISA Kit

MBS2020489

24-Strip-Wells

255 USD

ELISA Kit

Inducible T-Cell Co Stimulator (ICOS) ELISA Kit

[Inducible T-Cell Co Stimulator (ICOS)]

[CD278; AILIM; Inducible T-Cell Costimulator; Activation-Inducible Lymphocyte Immunomediatory Molecule]

[inducible T-cell co-stimulator; Inducible T-cell costimulator; inducible T-cell costimulator; inducible T-cell co-stimulator; Activation-inducible lymphocyte immunomediatory molecule; CD_antigen: CD278]

[ICOS]

[ICOS; ICOS; AILIM; CD278; CVID1; AILIM]

ICOS_HUMAN

Human

199

This assay has high sensitivity and excellent specificity for detection of Inducible T-Cell Co Stimulator (ICOS). No significant cross-reactivity or interference between Inducible T-Cell Co Stimulator (ICOS) and analogues was observed.

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Assay Type: Double-antibody Sandwich. Samples: Serum, Plasma, Urine, Cell lysates and Other Biological Fluids. Detection Range: 15.6-1,000pg/mL. Sensitivity: < 5.8pg/mL

Application: Enzyme-linked immunosorbent assay for Antigen Detection. Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Inducible T-Cell Co Stimulator (ICOS) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Inducible T-Cell Co Stimulator (ICOS) were tested on 3 different plates, 8 replicates in each plate. CV(%) =: SD/meanX100. Intra-Assay: CV<10%. Inter-Assay: CV<12%

Signal transduction; Tumor immunity; Infection immunity; Immune molecule

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Inducible T-Cell Co Stimulator (ICOS). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Inducible T-Cell Co Stimulator (ICOS). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Inducible T-Cell Co Stimulator (ICOS), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Inducible T-Cell Co Stimulator (ICOS) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

9968296

CAC06612.1

Q9Y6W8

19,127 Da

Adaptive Immune System Pathway (366160); Cell Adhesion Molecules (CAMs) Pathway (83069); Cell Adhesion Molecules (CAMs) Pathway (480); Costimulation By The CD28 Family Pathway (119552); Immune System Pathway (106386); Intestinal Immune Network For IgA Production Pathway (128760); Intestinal Immune Network For IgA Production Pathway (128670); Primary Immunodeficiency Pathway (83125); Primary Immunodeficiency Pathway (537); T Cell Receptor Signaling Pathway (83080)

The protein encoded by this gene belongs to the CD28 and CTLA-4 cell-surface receptor family. It forms homodimers and plays an important role in cell-cell signaling, immune responses, and regulation of cell proliferation. [provided by RefSeq, Jul 2008]

ICOS: Enhances all basic T-cell responses to a foreign antigen, namely proliferation, secretion of lymphokines, up- regulation of molecules that mediate cell-cell interaction, and effective help for antibody secretion by B-cells. Essential both for efficient interaction between T and B-cells and for normal antibody responses to T-cell dependent antigens. Does not up- regulate the production of interleukin-2, but superinduces the synthesis of interleukin-10. Prevents the apoptosis of pre- activated T-cells. Plays a critical role in CD40-mediated class switching of immunoglobin isotypes. Defects in ICOS are the cause of immunodeficiency common variable type 1 (CVID1). CVID1 is a primary immunodeficiency characterized by antibody deficiency, hypogammaglobulinemia, recurrent bacterial infections and an inability to mount an antibody response to antigen. The defect results from a failure of B-cell differentiation and impaired secretion of immunoglobulins; the numbers of circulating B-cells is usually in the normal range, but can be lo. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Membrane protein, integral. Chromosomal Location of Human Ortholog: 2q33. Cellular Component: integral to plasma membrane; plasma membrane; extracellular region; external side of plasma membrane. Biological Process: cell-cell adhesion; T cell tolerance induction; T cell costimulation; immune response. Disease: Immunodeficiency, Common Variable, 2; Immunodeficiency, Common Variable, 1

24-Strip-Wells

255 USD

48-Strip-Wells

455

96-Strip-Wells

610

5x96-Strip-Wells

2470

10x96-Strip-Wells

4445