ELISA/assay

Carnitine Palmitoyltransferase 1A, Liver (CPT1A) ELISA Kit

MBS2020426

48-Strip-Wells

605 USD

ELISA Kit

Carnitine Palmitoyltransferase 1A, Liver (CPT1A) ELISA Kit

Carnitine Palmitoyltransferase 1A, Liver (CPT1A)

CPT1-L; Carnitine O-palmitoyltransferase I, liver isoform

carnitine palmitoyltransferase 1A (liver), isoform CRA_a; Carnitine O-palmitoyltransferase 1, liver isoform; carnitine O-palmitoyltransferase 1, liver isoform; carnitine palmitoyltransferase 1A (liver); Carnitine O-palmitoyltransferase I, liver isoform; CPT I; CPTI-L; Carnitine palmitoyltransferase 1A

CPT1A

CPT1A; CPT1A; CPT1; CPT1-L; L-CPT1; CPT1; CPT1-L; CPT I; CPTI-L

CPT1A_HUMAN

Rat

773

This assay has high sensitivity and excellent specificity for detection of Carnitine Palmitoyltransferase 1A, Liver (CPT1A). No significant cross-reactivity or interference between Carnitine Palmitoyltransferase 1A, Liver (CPT1A) and analogues was observed.

For unopened kit: All the reagents should be kept according to the labels on vials. The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 degree C upon receipt while the others should be at 4 degree C. For opened kit: When the kit is opened, the remaining reagents still need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and reseal along entire edge of zip-seal. The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Samples: Serum, plasma, tissue homogenates and other biological fluids. Assay Type: Sandwich. Detection Range: 3.13-200ng/mL. Sensitivity: Typically less than 1.22ng/mL.

Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Carnitine Palmitoyltransferase 1A, Liver (CPT1A) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Carnitine Palmitoyltransferase 1A, Liver (CPT1A) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 . Intra-Assay: CV<10% . Inter-Assay: CV<12% . Assay Procedure Summary: 1. Prepare all reagents, samples and standards;. 2. Add 100uL standard or sample to each well. Incubate 2 hours at 37 degree C;. 3. Aspirate and add 100uL prepared Detection Reagent A. Incubate 1 hour at 37 degree C;. 4. Aspirate and wash 3 times;. 5. Add 100uL prepared Detection Reagent B. Incubate 30 minutes at 37 degree C;. 6. Aspirate and wash 5 times;. 7. Add 90uL Substrate Solution. Incubate 15-25 minutes at 37 degree C;. 8. Add 50uL Stop Solution. Read at 450nm immediately.

Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Carnitine Palmitoyltransferase 1A, Liver (CPT1A). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Carnitine Palmitoyltransferase 1A, Liver (CPT1A). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Carnitine Palmitoyltransferase 1A, Liver (CPT1A), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Carnitine Palmitoyltransferase 1A, Liver (CPT1A) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

119595128

EAW74722.1

P50416

86,239 Da

AMPK Signaling Pathway (198868); AMPK Signaling Pathway (989139); AMPK Signaling Pathway (992181); Adipocytokine Signaling Pathway (83093); Adipocytokine Signaling Pathway (505); Circadian Clock Pathway (187173); FOXA2 And FOXA3 Transcription Factor Networks Pathway (137911); Fatty Acid Beta Oxidation Pathway (198865); Fatty Acid Degradation Pathway (82935); Fatty Acid Degradation Pathway (296)

The mitochondrial oxidation of long-chain fatty acids is initiated by the sequential action of carnitine palmitoyltransferase I (which is located in the outer membrane and is detergent-labile) and carnitine palmitoyltransferase II (which is located in the inner membrane and is detergent-stable), together with a carnitine-acylcarnitine translocase. CPT I is the key enzyme in the carnitine-dependent transport across the mitochondrial inner membrane and its deficiency results in a decreased rate of fatty acid beta-oxidation. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

CPT1A: Belongs to the carnitine/choline acetyltransferase family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Membrane protein, multi-pass; Membrane protein, integral; Mitochondrial; Transferase; Lipid Metabolism - fatty acid; EC 2.3.1.21. Chromosomal Location of Human Ortholog: 11q13.2. Cellular Component: mitochondrial outer membrane; mitochondrion; membrane; mitochondrial inner membrane; integral to mitochondrial outer membrane. Molecular Function: identical protein binding; carnitine O-palmitoyltransferase activity. Biological Process: circadian rhythm; response to drug; eating behavior; glucose metabolic process; carnitine shuttle; long-chain fatty acid metabolic process; cellular lipid metabolic process; response to organic cyclic substance; positive regulation of fatty acid beta-oxidation; triacylglycerol metabolic process; fatty acid beta-oxidation; epithelial cell differentiation; carnitine metabolic process; regulation of insulin secretion; protein homooligomerization. Disease: Carnitine Palmitoyltransferase I Deficiency

48-Strip-Wells

605 USD

96-Strip-Wells

820

5x96-Strip-Wells

3220

10x96-Strip-Wells

5795