Neuregulin 4 (NRG4) ELISA Kit | MyBioSource MBS2020412 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Neuregulin 4 (NRG4) ELISA Kit

catalog :

MBS2020412

quantity :

48-Strip-Wells

price :

575 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS2020412

products type :

ELISA Kit

products full name :

Neuregulin 4 (NRG4) ELISA Kit

products short name :

Neuregulin 4 (NRG4)

products name syn :

HRG4; Pro-NRG4; Pro-neuregulin-4, membrane-bound isoform

other names :

Neuregulin 4; Pro-neuregulin-4, membrane-bound isoform; pro-neuregulin-4, membrane-bound isoform; neuregulin 4

products gene name :

NRG4

other gene names :

NRG4; NRG4; HRG4; Pro-NRG4; NRG-4

uniprot entry name :

NRG4_HUMAN

reactivity :

Mouse

sequence length :

115

specificity :

This assay has high sensitivity and excellent specificity for detection of Neuregulin 4 (NRG4). No significant cross-reactivity or interference between Neuregulin 4 (NRG4) and analogues was observed.

storage stability :

For unopened kit: All the reagents should be kept according to the labels on vials. The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 degree C upon receipt while the others should be at 4 degree C. For opened kit: When the kit is opened, the remaining reagents still need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and reseal along entire edge of zip-seal. The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

other info1 :

Samples: Serum, plasma, tissue homogenates and other biological fluids. Assay Type: Sandwich. Detection Range: 0.313-20ng/mL. Sensitivity: Typically less than 0.118ng/mL.

other info2 :

Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Neuregulin 4 (NRG4) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Neuregulin 4 (NRG4) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 . Intra-Assay: CV<10% . Inter-Assay: CV<12% . Assay Procedure Summary: 1. Prepare all reagents, samples and standards;. 2. Add 100uL standard or sample to each well. Incubate 2 hours at 37 degree C;. 3. Aspirate and add 100uL prepared Detection Reagent A. Incubate 1 hour at 37 degree C;. 4. Aspirate and wash 3 times;. 5. Add 100uL prepared Detection Reagent B. Incubate 30 minutes at 37 degree C;. 6. Aspirate and wash 5 times;. 7. Add 90uL Substrate Solution. Incubate 15-25 minutes at 37 degree C;. 8. Add 50uL Stop Solution. Read at 450nm immediately.

products description :

Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Neuregulin 4 (NRG4). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Neuregulin 4 (NRG4). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Neuregulin 4 (NRG4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Neuregulin 4 (NRG4) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

ncbi gi num :

17068379

ncbi acc num :

AAH17568.1

uniprot acc num :

Q8WWG1

ncbi mol weight :

12,722 Da

ncbi pathways :

Adaptive Immune System Pathway (366160); Constitutive PI3K/AKT Signaling In Cancer Pathway (685535); DAP12 Interactions Pathway (685549); DAP12 Signaling Pathway (685550); Disease Pathway (530764); Downstream Signal Transduction Pathway (106385); Downstream Signaling Events Of B Cell Receptor (BCR) Pathway (576250); Downstream Signaling Of Activated FGFR Pathway (160957); ErbB Receptor Signaling Network Pathway (138016); ErbB Signaling Pathway (198844)

ncbi summary :

The neuregulins, including NRG4, activate type-1 growth factor receptors (see EGFR; MIM 131550) to initiating cell-to-cell signaling through tyrosine phosphorylation (Harari et al., 1999 [PubMed 10348342]).[supplied by OMIM, Mar 2008]

uniprot summary :

NRG4: Low affinity ligand for the ERBB4 tyrosine kinase receptor. Concomitantly recruits ERBB1 and ERBB2 coreceptors, resulting in ligand-stimulated tyrosine phosphorylation and activation of the ERBB receptors. Does not bind to the ERBB1, ERBB2 and ERBB3 receptors. Belongs to the neuregulin family. Protein type: Membrane protein, integral. Chromosomal Location of Human Ortholog: 15q24.2. Cellular Component: extracellular space; extracellular region; integral to membrane; plasma membrane. Molecular Function: growth factor activity; receptor binding. Biological Process: epidermal growth factor receptor signaling pathway; fibroblast growth factor receptor signaling pathway; phosphoinositide-mediated signaling; nerve growth factor receptor signaling pathway; organ development; innate immune response

size1 :

48-Strip-Wells

price1 :

575 USD

size2 :

96-Strip-Wells

price2 :

785

size3 :

5x96-Strip-Wells

price3 :

3055

size4 :

10x96-Strip-Wells

price4 :

5495

more info or order :

MyBioSource product webpage