Protamine 2 (PRM2) ELISA Kit | MyBioSource MBS2020403 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Protamine 2 (PRM2) ELISA Kit

catalog :

MBS2020403

quantity :

48-Strip-Wells

price :

565 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS2020403

products type :

ELISA Kit

products full name :

Protamine 2 (PRM2) ELISA Kit

products short name :

Protamine 2 (PRM2)

products name syn :

CT94.2; Cancer/Testis Antigen Family 94,Member 2; Sperm histone P2; Sperm protamine P2

other names :

protamine 2; Protamine-2; Sperm histone P2; Sperm protamine P2

products gene name :

PRM2

other gene names :

PRM2; P2'

uniprot entry name :

PRM2_HUMAN

reactivity :

Human

sequence length :

102

specificity :

This assay has high sensitivity and excellent specificity for detection of Protamine 2 (PRM2). No significant cross-reactivity or interference between Protamine 2 (PRM2) and analogues was observed.

storage stability :

For unopened kit: All the reagents should be kept according to the labels on vials. The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 degree C upon receipt while the others should be at 4 degree C. For opened kit: When the kit is opened, the remaining reagents still need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and reseal along entire edge of zip-seal. The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

other info1 :

Samples: Tissue homogenates and other biological fluids. Assay Type: Sandwich. Detection Range: 0.156-10ng/mL. Sensitivity: Typically less than 0.057ng/mL.

other info2 :

Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Protamine 2 (PRM2) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Protamine 2 (PRM2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 . Intra-Assay: CV<10% . Inter-Assay: CV<12% . Assay Procedure Summary: 1. Prepare all reagents, samples and standards;. 2. Add 100uL standard or sample to each well. Incubate 2 hours at 37 degree C;. 3. Aspirate and add 100uL prepared Detection Reagent A. Incubate 1 hour at 37 degree C;. 4. Aspirate and wash 3 times;. 5. Add 100uL prepared Detection Reagent B. Incubate 30 minutes at 37 degree C;. 6. Aspirate and wash 5 times;. 7. Add 90uL Substrate Solution. Incubate 15-25 minutes at 37 degree C;. 8. Add 50uL Stop Solution. Read at 450nm immediately.

products description :

Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Protamine 2 (PRM2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Protamine 2 (PRM2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Protamine 2 (PRM2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Protamine 2 (PRM2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

ncbi gi num :

886877

ncbi acc num :

CAA87066.1

uniprot acc num :

P04554

ncbi mol weight :

17,106 Da

uniprot summary :

PRM2: Protamines substitute for histones in the chromatin of sperm during the haploid phase of spermatogenesis. They compact sperm DNA into a highly condensed, stable and inactive complex. Belongs to the protamine P2 family. Protein type: DNA-binding; Cancer Testis Antigen (CTA). Chromosomal Location of Human Ortholog: 16p13.2. Cellular Component: nucleoplasm; nucleosome; nucleus. Molecular Function: DNA binding. Biological Process: chromosome condensation; multicellular organismal development; spermatogenesis; cell differentiation; DNA packaging

size1 :

48-Strip-Wells

price1 :

565 USD

size2 :

96-Strip-Wells

price2 :

765

size3 :

5x96-Strip-Wells

price3 :

2975

size4 :

10x96-Strip-Wells

price4 :

5340

more info or order :

MyBioSource product webpage