ELISA/assay

Insulin Like Growth Factor Binding Protein, Acid Labile Subunit (IGFALS) ELISA Kit

MBS2020386

48-Strip-Wells

565 USD

ELISA Kit

Insulin Like Growth Factor Binding Protein, Acid Labile Subunit (IGFALS) ELISA Kit

Insulin Like Growth Factor Binding Protein, Acid Labile Subunit (IGFALS)

ALS

insulin-like growth factor-binding protein complex acid labile subunit isoform 1; Insulin-like growth factor-binding protein complex acid labile subunit; insulin-like growth factor-binding protein complex acid labile subunit; insulin-like growth factor binding protein, acid labile subunit

IGFALS

IGFALS; IGFALS; ALS; ACLSD; ALS; ALS

ALS_HUMAN

Human

643

This assay has high sensitivity and excellent specificity for detection of Insulin Like Growth Factor Binding Protein, Acid Labile Subunit (IGFALS). No significant cross-reactivity or interference between Insulin Like Growth Factor Binding Protein, Acid Labile Subunit (IGFALS) and analogues was observed.

For unopened kit: All the reagents should be kept according to the labels on vials. The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 degree C upon receipt while the others should be at 4 degree C. For opened kit: When the kit is opened, the remaining reagents still need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and reseal along entire edge of zip-seal. The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Samples: Serum, plasma, tissue homogenates and other biological fluids. Assay Type: Sandwich. Detection Range: 1.56-100ng/mL. Sensitivity: Typically less than 0.69ng/mL.

Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Insulin Like Growth Factor Binding Protein, Acid Labile Subunit (IGFALS) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Insulin Like Growth Factor Binding Protein, Acid Labile Subunit (IGFALS) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 . Intra-Assay: CV<10% . Inter-Assay: CV<12% . Assay Procedure Summary: 1. Prepare all reagents, samples and standards;. 2. Add 100uL standard or sample to each well. Incubate 2 hours at 37 degree C;. 3. Aspirate and add 100uL prepared Detection Reagent A. Incubate 1 hour at 37 degree C;. 4. Aspirate and wash 3 times;. 5. Add 100uL prepared Detection Reagent B. Incubate 30 minutes at 37 degree C;. 6. Aspirate and wash 5 times;. 7. Add 90uL Substrate Solution. Incubate 15-25 minutes at 37 degree C;. 8. Add 50uL Stop Solution. Read at 450nm immediately.

Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Insulin Like Growth Factor Binding Protein, Acid Labile Subunit (IGFALS). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Insulin Like Growth Factor Binding Protein, Acid Labile Subunit (IGFALS). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Insulin Like Growth Factor Binding Protein, Acid Labile Subunit (IGFALS), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Insulin Like Growth Factor Binding Protein, Acid Labile Subunit (IGFALS) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

225579152

NP_001139478.1

NM_001146006.1

P35858

70,236 Da

Metabolism Of Proteins Pathway (106230); Regulation Of Insulin-like Growth Factor (IGF) Transport And Uptake By Insulin-like Growth Factor Binding Proteins (IGFBPs) Pathway (105908)

The protein encoded by this gene is a serum protein that binds insulin-like growth factors, increasing their half-life and their vascular localization. Production of the encoded protein, which contains twenty leucine-rich repeats, is stimulated by growth hormone. Defects in this gene are a cause of acid-labile subunit deficiency, which maifests itself in a delayed and slow puberty. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Mar 2009]

IGFALS: Involved in protein-protein interactions that result in protein complexes, receptor-ligand binding or cell adhesion. Protein type: Secreted; Cell adhesion; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 16p13.3. Cellular Component: nucleoplasm; extracellular space; extracellular region. Molecular Function: insulin-like growth factor binding. Biological Process: cellular protein metabolic process; signal transduction; cell adhesion. Disease: Acid-labile Subunit Deficiency

48-Strip-Wells

565 USD

96-Strip-Wells

765

5x96-Strip-Wells

2975

10x96-Strip-Wells

5340