ELISA/assay

Immunoglobulin E (IgE) ELISA Kit

MBS2020348

24-Strip-Wells

265 USD

ELISA Kit

Immunoglobulin E (IgE) ELISA Kit

[Immunoglobulin E (IgE)]

[IGHE; Immunoglobulin Heavy Constant Epsilon; Ig epsilon chain C region]

[IgE]

Rat

This assay has high sensitivity and excellent specificity for detection of Immunoglobulin E (IgE). No significant cross-reactivity or interference between Immunoglobulin E (IgE) and analogues was observed.

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Assay Type: Double-antibody Sandwich. Samples: Serum, Plasma, and Other Biological Fluids. Detection Range: 31.2-2000pg/mL. Sensitivity: < 14.2pg/mL

Application: Enzyme-linked immunosorbent assay for Antigen Detection. Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Immunoglobulin E (IgE) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Immunoglobulin E (IgE) were tested on 3 different plates, 8 replicates in each plate. CV(%) =: SD/meanX100. Intra-Assay: CV<10%. Inter-Assay: CV<12%

Infection immunity; Immune molecule; Hematology; Autoimmunity

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Immunoglobulin E (IgE). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Immunoglobulin E (IgE). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Immunoglobulin E (IgE), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Immunoglobulin E (IgE) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

24-Strip-Wells

265 USD

48-Strip-Wells

490

96-Strip-Wells

655

5x96-Strip-Wells

2660

10x96-Strip-Wells

4795