ELISA/assay

Sodium/Hydrogen Exchanger 1 (NHE1) ELISA Kit

MBS2020325

48-Strip-Wells

565 USD

ELISA Kit

Sodium/Hydrogen Exchanger 1 (NHE1) ELISA Kit

Sodium/Hydrogen Exchanger 1 (NHE1)

SLC9A1; APNH; Solute Carrier Family 9 Member 1,Sodium/Hydrogen Exchanger; Antiporter,Na+/H+,Amiloride Sensitive; Na(+)/H(+) antiporter, amiloride-sensitive

sodium/hydrogen exchanger 1; Sodium/hydrogen exchanger 1; sodium/hydrogen exchanger 1; solute carrier family 9, subfamily A (NHE1, cation proton antiporter 1), member 1; APNH; Na(+)/H(+) antiporter, amiloride-sensitive; Na(+)/H(+) exchanger 1; NHE-1; Solute carrier family 9 member 1

NHE1

SLC9A1; SLC9A1; APNH; NHE1; LIKNS; NHE-1; PPP1R143; APNH1; NHE1; NHE-1

SL9A1_HUMAN

Human

815

This assay has high sensitivity and excellent specificity for detection of Sodium/Hydrogen Exchanger 1 (NHE1). No significant cross-reactivity or interference between Sodium/Hydrogen Exchanger 1 (NHE1) and analogues was observed.

For unopened kit: All the reagents should be kept according to the labels on vials. The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 degree C upon receipt while the others should be at 4 degree C. For opened kit: When the kit is opened, the remaining reagents still need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and reseal along entire edge of zip-seal. The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Samples: Tissue homogenates, Cell lysates and other biological fluids. Assay Type: Sandwich. Detection Range: 0.156-10ng/mL. Sensitivity: Typically less than 0.054ng/mL.

Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Sodium/Hydrogen Exchanger 1 (NHE1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Sodium/Hydrogen Exchanger 1 (NHE1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 . Intra-Assay: CV<10% . Inter-Assay: CV<12% . Assay Procedure Summary: 1. Prepare all reagents, samples and standards;. 2. Add 100uL standard or sample to each well. Incubate 2 hours at 37 degree C;. 3. Aspirate and add 100uL prepared Detection Reagent A. Incubate 1 hour at 37 degree C;. 4. Aspirate and wash 3 times;. 5. Add 100uL prepared Detection Reagent B. Incubate 30 minutes at 37 degree C;. 6. Aspirate and wash 5 times;. 7. Add 90uL Substrate Solution. Incubate 15-25 minutes at 37 degree C;. 8. Add 50uL Stop Solution. Read at 450nm immediately.

Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Sodium/Hydrogen Exchanger 1 (NHE1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Sodium/Hydrogen Exchanger 1 (NHE1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Sodium/Hydrogen Exchanger 1 (NHE1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Sodium/Hydrogen Exchanger 1 (NHE1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

27777632

NP_003038.2

NM_003047.4

P19634

61,013 Da

Adrenergic Signaling In Cardiomyocytes Pathway (908257); Adrenergic Signaling In Cardiomyocytes Pathway (909696); Bile Secretion Pathway (193146); Bile Secretion Pathway (193095); Cardiac Muscle Contraction Pathway (93344); Cardiac Muscle Contraction Pathway (93992); Defective B3GAT3 Causes JDSSDHD Pathway (1127588); Defective B4GALT1 Causes B4GALT1-CDG (CDG-2d) Pathway (1127592); Defective B4GALT7 Causes EDS, Progeroid Type Pathway (1127587); Defective CHST14 Causes EDS, Musculocontractural Type Pathway (1127591)

This gene encodes a Na+/H+ antiporter that is a member of the solute carrier family 9. The encoded protein is a plasma membrane transporter that is expressed in the kidney and intestine. This protein plays a central role in regulating pH homeostasis, cell migration and cell volume. This protein may also be involved in tumor growth. [provided by RefSeq, Sep 2011]

NHE1: sodium/hydrogen exchanger 1. Belongs to the Na(+)/H(+) exchanger family. Involved in pH regulation to eliminate acids generated by active metabolism or to counter adverse environmental conditions. Major proton extruding system driven by the inward sodium ion chemical gradient. Protein type: Motility/polarity/chemotaxis; Membrane protein, integral; Transporter; Membrane protein, multi-pass; Transporter, SLC family. Chromosomal Location of Human Ortholog: 1p36.1-p35. Cellular Component: endoplasmic reticulum membrane; focal adhesion; cell surface; mitochondrion; integral to plasma membrane; endoplasmic reticulum; basolateral plasma membrane; T-tubule; integral to membrane; lipid raft; nucleoplasm; lamellipodium; perinuclear region of cytoplasm; apical plasma membrane; cytoplasm; plasma membrane. Molecular Function: calmodulin binding; protein binding, bridging; protein binding; phosphatidylinositol-4,5-bisphosphate binding; protein complex scaffold; solute:hydrogen antiporter activity; calcium-dependent protein binding; sodium:hydrogen antiporter activity; protein phosphatase 2B binding. Biological Process: response to drug; cellular sodium ion homeostasis; cell migration; glycosaminoglycan metabolic process; positive regulation of NFAT protein import into nucleus; pathogenesis; maintenance of cell polarity; positive regulation of cell growth; cardiac muscle cell differentiation; protein oligomerization; hyaluronan catabolic process; cellular response to insulin stimulus; regulation of stress fiber formation; regulation of intracellular pH; regulation of focal adhesion formation; carbohydrate metabolic process; regulation of pH; positive regulation of transcription from RNA polymerase II promoter; ion transport; response to acidity; regulation of sensory perception of pain; positive regulation of action potential; cell growth; transmembrane transport; hyaluronan metabolic process; negative regulation of apoptosis. Disease: Lichtenstein-knorr Syndrome

48-Strip-Wells

565 USD

96-Strip-Wells

765

5x96-Strip-Wells

2975

10x96-Strip-Wells

5340