Bone Morphogenetic Protein 2 (BMP2) ELISA Kit | MyBioSource MBS2020318 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Bone Morphogenetic Protein 2 (BMP2) ELISA Kit

catalog :

MBS2020318

quantity :

24-Strip-Wells

price :

285 USD

more info or order :

MyBioSource product webpage

image

image 1 :

product information

catalog number :

MBS2020318

products type :

ELISA Kit

products full name :

Bone Morphogenetic Protein 2 (BMP2) ELISA Kit

products short name :

[Bone Morphogenetic Protein 2 (BMP2)]

products name syn :

[BMP2A; BMP-2A; Hemochromatosis Modifier]

other names :

[bone morphogenetic protein 2 preproprotein; Bone morphogenetic protein 2; bone morphogenetic protein 2; bone morphogenetic protein 2; Bone morphogenetic protein 2A; BMP-2A]

products gene name :

[BMP2]

other gene names :

[BMP2; BMP2; BDA2; BMP2A; BMP2A; BMP-2; BMP-2A]

uniprot entry name :

BMP2_HUMAN

reactivity :

Bovine

sequence length :

396

specificity :

This assay has high sensitivity and excellent specificity for detection of Bone Morphogenetic Protein 2 (BMP2). No significant cross-reactivity or interference between Bone Morphogenetic Protein 2 (BMP2) and analogues was observed.

storage stability :

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

image1 heading :

Typical Testing Data/Standard Curve

other info1 :

Assay Type: Double-antibody Sandwich. Samples: Serum, Plasma, Tissue homogenates and Other Biological Fluids. Detection Range: 15.6-1000pg/mL. Sensitivity: < 6.3pg/mL

other info2 :

Application: Enzyme-linked immunosorbent assay for Antigen Detection. Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Bone Morphogenetic Protein 2 (BMP2) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Bone Morphogenetic Protein 2 (BMP2) were tested on 3 different plates, 8 replicates in each plate. CV(%) =: SD/meanX100. Intra-Assay: CV<10%. Inter-Assay: CV<12%

products categories :

Developmental science; Bone metabolism

products description :

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Bone Morphogenetic Protein 2 (BMP2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Bone Morphogenetic Protein 2 (BMP2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Bone Morphogenetic Protein 2 (BMP2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Bone Morphogenetic Protein 2 (BMP2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

ncbi gi num :

4557369

ncbi acc num :

NP_001191.1

ncbi gb acc num :

NM_001200.2

uniprot acc num :

P12643

ncbi mol weight :

44,702 Da

ncbi pathways :

Adipogenesis Pathway (198832); BDNF Signaling Pathway (712093); BMP Signaling Pathway (1084755); BMP Signaling Pathway (1108218); BMP Signalling And Regulation Pathway (198910); Basal Cell Carcinoma Pathway (83113); Basal Cell Carcinoma Pathway (525); Cytokine-cytokine Receptor Interaction Pathway (83051); Cytokine-cytokine Receptor Interaction Pathway (460); Elastic Fibre Formation Pathway (730310)

ncbi summary :

The protein encoded by this gene belongs to the transforming growth factor-beta (TGFB) superfamily. The encoded protein acts as a disulfide-linked homodimer and induces bone and cartilage formation. [provided by RefSeq, Jul 2008]

uniprot summary :

Induces cartilage and bone formation (PubMed:3201241). Stimulates the differentiation of myoblasts into osteoblasts via the EIF2AK3-EIF2A- ATF4 pathway. BMP2 activation of EIF2AK3 stimulates phosphorylation of EIF2A which leads to increased expression of ATF4 which plays a central role in osteoblast differentiation. In addition stimulates TMEM119, which upregulates the expression of ATF4 (PubMed:24362451).

size1 :

24-Strip-Wells

price1 :

285 USD

size2 :

48-Strip-Wells

price2 :

525

size3 :

96-Strip-Wells

price3 :

715

size4 :

5x96-Strip-Wells

price4 :

2895

size5 :

10x96-Strip-Wells

price5 :

5255

more info or order :

MyBioSource product webpage