ELISA/assay

Semaphorin 3A (SEMA3A) ELISA Kit

MBS2020300

48-Strip-Wells

610 USD

ELISA Kit

Semaphorin 3A (SEMA3A) ELISA Kit

Semaphorin 3A (SEMA3A)

SEMAL; Hsema-I; Hsema-III; SEMA1; SEMAD; SEMAIII; SemD; Coll-1; Sema Domain,Immunoglobulin Domain(Ig),Transmembrane Domain(TM)and Short Cytoplasmic Domain 3A

semaphorin-3A; Semaphorin-3A; semaphorin-3A; sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3A; Semaphorin III; Sema III

SEMA3A

SEMA3A; SEMA3A; HH16; SemD; COLL1; SEMA1; SEMAD; SEMAL; coll-1; Hsema-I; SEMAIII; Hsema-III; SEMAD; Sema III

SEM3A_HUMAN

Human

771

This assay has high sensitivity and excellent specificity for detection of Semaphorin 3A (SEMA3A). No significant cross-reactivity or interference between Semaphorin 3A (SEMA3A) and analogues was observed.

For unopened kit: All the reagents should be kept according to the labels on vials. The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 degree C upon receipt while the others should be at 4 degree C. For opened kit: When the kit is opened, the remaining reagents still need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and reseal along entire edge of zip-seal. The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Samples: Serum, plasma, tissue homogenates and other biological fluids. Assay Type: Sandwich. Detection Range: 0.156-10ng/mL. Sensitivity: Typically less than 0.068ng/mL.

Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Semaphorin 3A (SEMA3A) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Semaphorin 3A (SEMA3A) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 . Intra-Assay: CV<10% . Inter-Assay: CV<12% . Assay Procedure Summary: 1. Prepare all reagents, samples and standards;. 2. Add 100uL standard or sample to each well. Incubate 2 hours at 37 degree C;. 3. Aspirate and add 100uL prepared Detection Reagent A. Incubate 1 hour at 37 degree C;. 4. Aspirate and wash 3 times;. 5. Add 100uL prepared Detection Reagent B. Incubate 30 minutes at 37 degree C;. 6. Aspirate and wash 5 times;. 7. Add 90uL Substrate Solution. Incubate 15-25 minutes at 37 degree C;. 8. Add 50uL Stop Solution. Read at 450nm immediately.

Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Semaphorin 3A (SEMA3A). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Semaphorin 3A (SEMA3A). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Semaphorin 3A (SEMA3A), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Semaphorin 3A (SEMA3A) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

5174673

NP_006071.1

NM_006080.2

Q14563

88,889 Da

Axon Guidance Pathway (83065); Axon Guidance Pathway (476); Axon Guidance Pathway (105688); CRMPs In Sema3A Signaling Pathway (119525); Developmental Biology Pathway (477129); SEMA3A-Plexin Repulsion Signaling By Inhibiting Integrin Adhesion Pathway (119523); Sema3A PAK Dependent Axon Repulsion Pathway (119524); Semaphorin Interactions Pathway (119519)

This gene is a member of the semaphorin family and encodes a protein with an Ig-like C2-type (immunoglobulin-like) domain, a PSI domain and a Sema domain. This secreted protein can function as either a chemorepulsive agent, inhibiting axonal outgrowth, or as a chemoattractive agent, stimulating the growth of apical dendrites. In both cases, the protein is vital for normal neuronal pattern development. Increased expression of this protein is associated with schizophrenia and is seen in a variety of human tumor cell lines. Also, aberrant release of this protein is associated with the progression of Alzheimer's disease. [provided by RefSeq, Jul 2008]

SEMA3A: Induces the collapse and paralysis of neuronal growth cones. Could serve as a ligand that guides specific growth cones by a motility-inhibiting mechanism. Binds to the complex neuropilin-1/plexin-1. Belongs to the semaphorin family. Protein type: Secreted; Motility/polarity/chemotaxis; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 7p12.1. Cellular Component: membrane; axon; dendrite; extracellular region. Molecular Function: chemorepellent activity; receptor activity; semaphorin receptor binding. Biological Process: facial nerve structural organization; trigeminal nerve structural organization; axon guidance; apoptosis; regulation of heart rate; dendrite morphogenesis; olfactory bulb development; neuron migration; axonal fasciculation; negative chemotaxis; negative regulation of axon extension involved in axon guidance; axon extension involved in axon guidance; gonadotrophin-releasing hormone neuronal migration to the hypothalamus; sympathetic nervous system development; branchiomotor neuron axon guidance; regulation of axon extension involved in axon guidance; nerve development; sensory system development. Disease: Hypogonadotropic Hypogonadism 16 With Or Without Anosmia

48-Strip-Wells

610 USD

96-Strip-Wells

830

5x96-Strip-Wells

3265

10x96-Strip-Wells

5870