ELISA/assay

Adenosine Deaminase (ADA) ELISA Kit

MBS2020269

24-Strip-Wells

260 USD

ELISA Kit

Adenosine Deaminase (ADA) ELISA Kit

[Adenosine Deaminase (ADA)]

[Adenosine Aminohydrolase]

[adenosine deaminase; Adenosine deaminase; adenosine deaminase; adenosine deaminase; Adenosine aminohydrolase]

[ADA]

[ADA; ADA; ADA1]

ADA_HUMAN

Human

363

This assay has high sensitivity and excellent specificity for detection of ACVR2B. No significant cross-reactivity or interference between ACVR2B and analogues was observed.

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Samples: Mouse Tissue Homogenates, Cell Lysates, Cell Culture Supernates And Other Biological Fluids. Assay Type: Quantitative Sandwich. Detection Range: 0.156-10ng/mL. Sensitivity: 0.056ng/mL.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level ACVR2B were tested 20 times on one plate, respectively. Intra-Assay: CV<10%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level ACVR2B were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%

Enzyme Tumor immunity; Infection immunity

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of ACVR2B in mouse tissue homogenates, cell lysates, cell culture supernates and other biological fluids. Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to ACVR2B. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to ACVR2B. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain ACVR2B, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of ACVR2B in the samples is then determined by comparing the O.D. of the samples to the standard curve.

47078295

NP_000013.2

NM_000022.2

P00813

40,764 Da

C-MYB Transcription Factor Network Pathway (138073); Metabolic Pathways (132956); Metabolism Pathway (477135); Metabolism Of Nucleotides Pathway (106263); Primary Immunodeficiency Pathway (83125); Primary Immunodeficiency Pathway (537); Purine Metabolism Pathway (82944); Purine Metabolism Pathway (106265); Purine Metabolism Pathway (307); Purine Salvage Pathway (106273)

This gene encodes an enzyme that catalyzes the hydrolysis of adenosine to inosine. Various mutations have been described for this gene and have been linked to human diseases. Deficiency in this enzyme causes a form of severe combined immunodeficiency disease (SCID), in which there is dysfunction of both B and T lymphocytes with impaired cellular immunity and decreased production of immunoglobulins, whereas elevated levels of this enzyme have been associated with congenital hemolytic anemia. [provided by RefSeq, Jul 2008]

ADA: a enzyme that converts adenosine + H2O into inosine + NH3. Found in all tissues, occurs in large amounts in T-lymphocytes and, at the time of weaning, in gastrointestinal tissues. Genetic ADA deficiencies are a cause of autosomal recessive severe combined immuno-deficiency (SCID). Hereditary hemolytic anemia is caused by expression levels in erythrocytes 50-70 times greater than the norm. Protein type: Nucleotide Metabolism - purine; Hydrolase; EC 3.5.4.4. Chromosomal Location of Human Ortholog: 20q13.12. Cellular Component: dendrite cytoplasm; extracellular space; cell surface; cell soma; membrane; lysosome; cytoplasm; plasma membrane; cytosol; cell junction; external side of plasma membrane. Molecular Function: adenosine deaminase activity; protein binding; zinc ion binding; purine nucleoside binding. Biological Process: negative regulation of circadian sleep/wake cycle, non-REM sleep; adenosine catabolic process; T cell activation; deoxyadenosine catabolic process; response to morphine; positive regulation of calcium-mediated signaling; purine salvage; positive regulation of T cell differentiation in the thymus; histamine secretion; purine ribonucleoside monophosphate biosynthetic process; response to vitamin E; regulation of cell-cell adhesion mediated by integrin; positive regulation of T cell receptor signaling pathway; negative regulation of mature B cell apoptosis; positive regulation of germinal center formation; positive regulation of B cell proliferation; hypoxanthine salvage; positive regulation of smooth muscle contraction; negative regulation of adenosine receptor signaling pathway; embryonic gut development; placenta development; aging; Peyer s patch development; response to drug; dATP catabolic process; nucleobase, nucleoside and nucleotide metabolic process; positive regulation of heart rate; liver development; negative regulation of leukocyte migration; purine base metabolic process; trophectodermal cell differentiation; purine nucleotide salvage; xanthine biosynthetic process; response to hydrogen peroxide; negative regulation of inflammatory response; response to hypoxia; inosine biosynthetic process; germinal center B cell differentiation; alveolus development; positive regulation of alpha-beta T cell differentiation. Disease: Severe Combined Immunodeficiency, Autosomal Recessive, T Cell-negative, B Cell-negative, Nk Cell-negative, Due To Adenosine Deaminase Deficiency

24-Strip-Wells

260 USD

48-Strip-Wells

475

96-Strip-Wells

640

5x96-Strip-Wells

2590

10x96-Strip-Wells

4670