ELISA/assay

Alpha-2-Macroglobulin (a2M) ELISA Kit

MBS2020230

24-Strip-Wells

230 USD

ELISA Kit

Alpha-2-Macroglobulin (a2M) ELISA Kit

[Alpha-2-Macroglobulin (a2M)]

[A2MG; A2mg; CPAMD5; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 5]

[alpha-2-macroglobulin; Alpha-2-macroglobulin; alpha-2-macroglobulin; alpha-2-macroglobulin; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 5]

[a2M]

[A2M; A2M; A2MD; CPAMD5; FWP007; S863-7; CPAMD5; Alpha-2-M]

A2MG_HUMAN

Mouse

1474

This assay has high sensitivity and excellent specificity for detection of 7-DHC. No significant cross-reactivity or interference between 7-DHC and analogues was observed.

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Samples: Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates And Other Biological Fluids. Assay Type: Quantitative Competitive. Detection Range: 2.47-200ng/mL. Sensitivity: 0.93ng/mL.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level 7-DHC were tested 20 times on one plate, respectively. Intra-Assay: CV<10%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level 7-DHC were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%

Tumor immunity; Infection immunity; Hematology

Intended Uses: The kit is a competitive inhibition enzyme immunoassay technique for the in vitro quantitative measurement of 7-DHC in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids. Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to 7-DHC has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled 7-DHC and unlabeled 7-DHC (Standards or samples) with the pre-coated antibody specific to 7-DHC. After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of 7-DHC in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of 7-DHC in the sample.

66932947

NP_000005.2

NM_000014.4

P01023

163,291 Da

Complement And Coagulation Cascades Pathway (198880); Complement And Coagulation Cascades Pathway (83073); Complement And Coagulation Cascades Pathway (484); Degradation Of The Extracellular Matrix Pathway (576263); Extracellular Matrix Organization Pathway (576262); Formation Of Fibrin Clot (Clotting Cascade) Pathway (106057); HDL-mediated Lipid Transport Pathway (106158); Hemostasis Pathway (106028); IL6-mediated Signaling Events Pathway (137932); Intrinsic Pathway (106059)

Alpha-2-macroglobulin is a protease inhibitor and cytokine transporter. It inhibits many proteases, including trypsin, thrombin and collagenase. A2M is implicated in Alzheimer disease (AD) due to its ability to mediate the clearance and degradation of A-beta, the major component of beta-amyloid deposits. [provided by RefSeq, Jul 2008]

A2M: Is able to inhibit all four classes of proteinases by a unique trapping mechanism. This protein has a peptide stretch, called the bait region which contains specific cleavage sites for different proteinases. When a proteinase cleaves the bait region, a conformational change is induced in the protein which traps the proteinase. The entrapped enzyme remains active against low molecular weight substrates (activity against high molecular weight substrates is greatly reduced). Following cleavage in the bait region a thioester bond is hydrolyzed and mediates the covalent binding of the protein to the proteinase. Belongs to the protease inhibitor I39 (alpha-2- macroglobulin) family. Protein type: Inhibitor; Secreted, signal peptide; Secreted. Chromosomal Location of Human Ortholog: 12p13.31. Cellular Component: extracellular region; cytosol. Molecular Function: serine-type endopeptidase inhibitor activity; protein binding; enzyme binding; protease binding; growth factor binding; interleukin-8 binding; interleukin-1 binding; calcium-dependent protein binding; tumor necrosis factor binding; receptor binding. Biological Process: negative regulation of complement activation, lectin pathway; extracellular matrix disassembly; platelet activation; extracellular matrix organization and biogenesis; regulation of small GTPase mediated signal transduction; platelet degranulation; small GTPase mediated signal transduction; stem cell differentiation; blood coagulation; blood coagulation, intrinsic pathway. Disease: Alzheimer Disease; Alpha-2-macroglobulin Deficiency

24-Strip-Wells

230 USD

48-Strip-Wells

380

96-Strip-Wells

505

5x96-Strip-Wells

2020

10x96-Strip-Wells

3640