Apolipoprotein M (APOM) ELISA Kit | MyBioSource MBS2020186 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Apolipoprotein M (APOM) ELISA Kit

catalog :

MBS2020186

quantity :

48-Strip-Wells

price :

565 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS2020186

products type :

ELISA Kit

products full name :

Apolipoprotein M (APOM) ELISA Kit

products short name :

Apolipoprotein M (APOM)

products name syn :

Apo-M; G3a; NG20; Protein G3a

other names :

Apolipoprotein M; Apolipoprotein M; apolipoprotein M; apolipoprotein M; Protein G3a

products gene name :

APOM

other gene names :

APOM; APOM; G3a; NG20; apo-M; HSPC336; G3A; NG20; Apo-M; ApoM

uniprot entry name :

APOM_HUMAN

reactivity :

Human

sequence length :

188

specificity :

This assay has high sensitivity and excellent specificity for detection of Apolipoprotein M (APOM). No significant cross-reactivity or interference between Apolipoprotein M (APOM) and analogues was observed.

storage stability :

For unopened kit: All the reagents should be kept according to the labels on vials. The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 degree C upon receipt while the others should be at 4 degree C. For opened kit: When the kit is opened, the remaining reagents still need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and reseal along entire edge of zip-seal. The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

other info1 :

Samples: Serum, plasma, tissue homogenates and other biological fluids. Assay Type: Sandwich. Detection Range: 0.313-20ng/mL. Sensitivity: Typically less than 0.137ng/mL.

other info2 :

Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Apolipoprotein M (APOM) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Apolipoprotein M (APOM) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 . Intra-Assay: CV<10% . Inter-Assay: CV<12% . Assay Procedure Summary: 1. Prepare all reagents, samples and standards;. 2. Add 100uL standard or sample to each well. Incubate 2 hours at 37 degree C;. 3. Aspirate and add 100uL prepared Detection Reagent A. Incubate 1 hour at 37 degree C;. 4. Aspirate and wash 3 times;. 5. Add 100uL prepared Detection Reagent B. Incubate 30 minutes at 37 degree C;. 6. Aspirate and wash 5 times;. 7. Add 90uL Substrate Solution. Incubate 15-25 minutes at 37 degree C;. 8. Add 50uL Stop Solution. Read at 450nm immediately.

products description :

Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Apolipoprotein M (APOM). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Apolipoprotein M (APOM). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Apolipoprotein M (APOM), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Apolipoprotein M (APOM) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

ncbi gi num :

18089092

ncbi acc num :

AAH20683.1

uniprot acc num :

O95445

ncbi mol weight :

13,051 Da

ncbi summary :

The protein encoded by this gene is an apolipoprotein and member of the lipocalin protein family. It is found associated with high density lipoproteins and to a lesser extent with low density lipoproteins and triglyceride-rich lipoproteins. The encoded protein is secreted through the plasma membrane but remains membrane-bound, where it is involved in lipid transport. Alternate splicing results in both coding and non-coding variants of this gene. [provided by RefSeq, Jan 2012]

uniprot summary :

APOM: Probably involved in lipid transport. Can bind sphingosine-1-phosphate, myristic acid, palmitic acid and stearic acid, retinol, all-trans-retinoic acid and 9-cis-retinoic acid. Plasma protein. Expressed in liver and kidney. Belongs to the calycin superfamily. Lipocalin family. Highly divergent. Protein type: Secreted; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 6p21.33. Cellular Component: integral to plasma membrane. Molecular Function: lipid transporter activity; antioxidant activity; phospholipid binding. Biological Process: cholesterol homeostasis; reverse cholesterol transport; response to glucose stimulus; lipoprotein metabolic process; cholesterol efflux

size1 :

48-Strip-Wells

price1 :

565 USD

size2 :

96-Strip-Wells

price2 :

765

size3 :

5x96-Strip-Wells

price3 :

2975

size4 :

10x96-Strip-Wells

price4 :

5340

more info or order :

MyBioSource product webpage