Human Neuromedin U (NMU) ELISA Kit | MyBioSource MBS2020184 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Neuromedin U (NMU) ELISA Kit

catalog :

MBS2020184

quantity :

48-Strip-Wells

price :

540 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS2020184

products type :

ELISA Kit

products full name :

Human Neuromedin U (NMU) ELISA Kit

products short name :

Neuromedin U (NMU)

other names :

neuromedin U; Neuromedin-U; neuromedin-U; prepro-NMU; neuromedin U

products gene name :

NMU

other gene names :

NMU; NMU; NmU-25

uniprot entry name :

NMU_HUMAN

reactivity :

Human

sequence length :

174

specificity :

This assay has high sensitivity and excellent specificity for detection of NMU. No significant cross-reactivity or interference between NMU and analogues was observed.

storage stability :

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

other info1 :

Samples: Human serum, plasma and other biological fluids. Assay Type: Competitive. Detection Range: 123.5-10,000pg/mL. The standard curve concentrations used for the ELISA's were 10,000pg/mL, 3,333.3pg/mL, 1,111.1pg/mL, 370.4pg/mL, 123.5pg/mL. Sensitivity: 48.3pg/mL.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level NMU were tested 20 times on one plate, respectively. Intra-Assay: CV<10%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level NMU were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%

products description :

Intended Uses: The kit is a competitive inhibition enzyme immunoassay technique for the in vitro quantitative measurement of NMU in human serum, plasma and other biological fluids. Principle of the Assay This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to NMU has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled NMU and unlabeled NMU (Standards or samples) with the pre-coated antibody specific to NMU. After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of NMU in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of NMU in the sample.

ncbi gi num :

609013

ncbi acc num :

CAA53619.1

uniprot acc num :

P48645

ncbi mol weight :

19,741 Da

ncbi pathways :

Class A/1 (Rhodopsin-like Receptors) Pathway (106357); G Alpha (i) Signalling Events Pathway (119550); G Alpha (q) Signalling Events Pathway (106043); GPCR Downstream Signaling Pathway (119548); GPCR Ligand Binding Pathway (161020); Gastrin-CREB Signalling Pathway Via PKC And MAPK (645295); Peptide Ligand-binding Receptors Pathway (106358); Signal Transduction Pathway (477114); Signaling By GPCR Pathway (106356)

ncbi summary :

This gene encodes a member of the neuromedin family of neuropeptides. The encoded protein is a precursor that is proteolytically processed to generate a biologically active neuropeptide that plays a role in pain, stress, immune-mediated inflammatory diseases and feeding regulation. Increased expression of this gene was observed in renal, pancreatic and lung cancers. Alternative splicing results in multiple transcript variants encoding different isoforms. Some of these isoforms may undergo similar processing to generate the mature peptide. [provided by RefSeq, Jul 2015]

uniprot summary :

NMU: Stimulates muscle contractions of specific regions of the gastrointestinal tract. In humans, NmU stimulates contractions of the ileum and urinary bladder. Belongs to the NmU family. Protein type: Secreted, signal peptide; Secreted. Chromosomal Location of Human Ortholog: 4q12. Cellular Component: extracellular region; terminal button. Molecular Function: neuromedin U receptor binding; receptor binding. Biological Process: regulation of smooth muscle contraction; gastric acid secretion; G-protein coupled receptor protein signaling pathway; eating behavior; neuropeptide signaling pathway; photoperiodism; digestion; positive regulation of hormone secretion; positive regulation of smooth muscle contraction; sensory perception of pain; signal transduction; positive regulation of synaptic transmission

size1 :

48-Strip-Wells

price1 :

540 USD

size2 :

96-Strip-Wells

price2 :

730

size3 :

5x96-Strip-Wells

price3 :

2830

size4 :

10x96-Strip-Wells

price4 :

5080

more info or order :

MyBioSource product webpage