ELISA/assay

D-Dopachrome Tautomerase (DDT) ELISA Kit

MBS2020153

24-Strip-Wells

270 USD

ELISA Kit

D-Dopachrome Tautomerase (DDT) ELISA Kit

[D-Dopachrome Tautomerase (DDT)]

[DDCT; D-Dopachrome Decarboxylase; Phenylpyruvate tautomerase II]

[D-dopachrome tautomerase; D-dopachrome decarboxylase; D-dopachrome tautomerase; Phenylpyruvate tautomerase II]

[DDT]

[DDT]

DOPD_HUMAN

Human

118

This assay has high sensitivity and excellent specificity for detection of DDT. No significant cross-reactivity or interference between DDT and analogues was observed.

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Samples: tissue homogenates, cell lysates and other biological fluids. Assay Type: Quantitative Sandwich. Detection Range: 0.312-20ng/mL. Sensitivity: <0.114ng/mL.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level DDT were tested 20 times on one plate, respectively. Intra-Assay: CV<10%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level DDT were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%

Enzyme & Kinase

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of DDT in human tissue homogenates, cell lysates and other biological fluids. Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to DDT. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to DDT. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain DDT, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of DDT in the samples is then determined by comparing the O.D. of the samples to the standard curve.

1805303

AAB41503.1

11,323 Da

DDT: Tautomerization of D-dopachrome with decarboxylation to give 5,6-dihydroxyindole (DHI). Belongs to the MIF family. Protein type: EC 4.1.1.84; Lyase. Chromosomal Location of Human Ortholog: 22q11.23. Cellular Component: cytoplasm. Molecular Function: D-dopachrome decarboxylase activity; dopachrome isomerase activity. Biological Process: melanin biosynthetic process

24-Strip-Wells

270 USD

48-Strip-Wells

495

96-Strip-Wells

665

5x96-Strip-Wells

2715

10x96-Strip-Wells

4895