Lysyl Oxidase Like Protein 1 (LOXL1) ELISA Kit | MyBioSource MBS2020148 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Lysyl Oxidase Like Protein 1 (LOXL1) ELISA Kit

catalog :

MBS2020148

quantity :

24-Strip-Wells

price :

270 USD

more info or order :

MyBioSource product webpage

image

image 1 :

product information

catalog number :

MBS2020148

products type :

ELISA Kit

products full name :

Lysyl Oxidase Like Protein 1 (LOXL1) ELISA Kit

products short name :

[Lysyl Oxidase Like Protein 1 (LOXL1)]

products name syn :

[LOL; LOXL; Lysyl oxidase homolog 1]

other names :

[lysyl oxidase homolog 1 preproprotein; Lysyl oxidase homolog 1; lysyl oxidase homolog 1; lysyl oxidase-like 1; Lysyl oxidase-like protein 1; LOL]

products gene name :

[LOXL1]

other gene names :

[LOXL1; LOXL1; LOL; LOXL; LOXL; LOL]

uniprot entry name :

LOXL1_HUMAN

reactivity :

Human

sequence length :

574

specificity :

This assay has high sensitivity and excellent specificity for detection of loricrin. No significant cross-reactivity or interference between loricrin and analogues was observed.

storage stability :

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

image1 heading :

Typical Testing Data/Standard Curve (for reference only)

other info1 :

Samples: Mouse Tissue Homogenates, Cell Lysates And Other Biological Fluids. Assay Type: Quantitative Sandwich. Detection Range: 0.156-10ng/mL. Sensitivity: 0.057ng/mL.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level loricrin were tested 20 times on one plate, respectively. Intra-Assay: CV<10%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level loricrin were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%

products categories :

Enzyme & Kinase

products description :

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of loricrin in mouse tissue homogenates, cell lysates and other biological fluids. Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to loricrin. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to loricrin. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain loricrin, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of loricrin in the samples is then determined by comparing the O.D. of the samples to the standard curve.

ncbi gi num :

67782346

ncbi acc num :

NP_005567.2

ncbi gb acc num :

NM_005576.2

uniprot acc num :

Q08397

ncbi mol weight :

63,110 Da

ncbi pathways :

Assembly Of Collagen Fibrils And Other Multimeric Structures Pathway (730306); Collagen Formation Pathway (645288); Crosslinking Of Collagen Fibrils Pathway (730308); Elastic Fibre Formation Pathway (730310); Extracellular Matrix Organization Pathway (576262)

ncbi summary :

This gene encodes a member of the lysyl oxidase gene family. The prototypic member of the family is essential to the biogenesis of connective tissue, encoding an extracellular copper-dependent amine oxidase that catalyses the first step in the formation of crosslinks in collagens and elastin. A highly conserved amino acid sequence at the C-terminus end appears to be sufficient for amine oxidase activity, suggesting that each family member may retain this function. The N-terminus is poorly conserved and may impart additional roles in developmental regulation, senescence, tumor suppression, cell growth control, and chemotaxis to each member of the family. [provided by RefSeq, Jul 2008]

uniprot summary :

LOXL1: Active on elastin and collagen substrates. Genetic variations in LOXL1 are a cause of susceptibility to exfoliation syndrome (XFS); also called exfoliation glaucoma (XFG). XFS is a disorder characterized by accumulation of abnormal fibrillar deposits in the anterior segment of the eye. In addition to being a cause of glaucoma and glaucomatous optic neuropathy, exfoliation syndrome has also been associated with lens zonule weakness, cataract formation, and systemic vascular complications due to deposition of exfoliation material in extraocular tissues. Susceptibility to exfoliation syndrome is conferred by a risk haplotype that includes two LOXL1 coding non-synonymous SNPs (Arg141Leu and Gly153Asp) and one intronic SNP. Arg141Leu and Gly153Asp are sufficient to confer disease susceptibility in some populations. Belongs to the lysyl oxidase family. Protein type: Oxidoreductase; Extracellular matrix; EC 1.4.3.-; Secreted; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 15q22. Cellular Component: extracellular matrix; extracellular space; extracellular region; acrosome; basement membrane. Molecular Function: copper ion binding; oxidoreductase activity, acting on the CH-NH2 group of donors, oxygen as acceptor. Biological Process: extracellular matrix organization and biogenesis; response to lipopolysaccharide; protein amino acid deamination. Disease: Exfoliation Syndrome

size1 :

24-Strip-Wells

price1 :

270 USD

size2 :

48-Strip-Wells

price2 :

495

size3 :

96-Strip-Wells

price3 :

665

size4 :

5x96-Strip-Wells

price4 :

2715

size5 :

10x96-Strip-Wells

price5 :

4895

more info or order :

MyBioSource product webpage