ELISA/assay

Gremlin 1 (GREM1) ELISA Kit

MBS2020142

24-Strip-Wells

270 USD

ELISA Kit

Gremlin 1 (GREM1) ELISA Kit

[Gremlin 1 (GREM1)]

[PIG2; CKTSF1B1; DAND2; IHG-2; DAND2; DRM; Increased in high glucose protein 2; Cell proliferation-inducing gene 2 protein; Cysteine knot superfamily 1, BMP antagonist 1]

[Gremlin 1; Gremlin-1; gremlin-1; gremlin 1; Cysteine knot superfamily 1, BMP antagonist 1; Down-regulated in Mos-transformed cells protein]

[GREM1]

[Grem1; Grem1; ld; Drm; Grem; Cktsf1b1; Cktsf1b1; Drm]

GREM1_MOUSE

Human

184

This assay has high sensitivity and excellent specificity for detection of Gremlin 1 (GREM1). No significant cross-reactivity or interference between Gremlin 1 (GREM1) and analogues was observed.

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Assay Type: Double-antibody Sandwich. Samples: Serum, Plasma, Urine, Tissue homogenates and Other Biological Fluids. Detection Range: 125-8,000pg/mL. Sensitivity: < 55pg/mL

Application: Enzyme-linked immunosorbent assay for Antigen Detection. Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Gremlin 1 (GREM1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Gremlin 1 (GREM1) were tested on 3 different plates, 8 replicates in each plate. CV(%) =: SD/meanX100. Intra-Assay: CV<10%. Inter-Assay: CV<12%

Signal transduction; Tumor immunity; Developmental science; Bone metabolism

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Gremlin 1 (GREM1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Gremlin 1 (GREM1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Gremlin 1 (GREM1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Gremlin 1 (GREM1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

15929743

AAH15293.1

O70326

20,710 Da

GREM1: Cytokine that may play an important role during carcinogenesis and metanephric kidney organogenesis, as a BMP antagonist required for early limb outgrowth and patterning in maintaining the FGF4-SHH feedback loop. Down-regulates the BMP4 signaling in a dose-dependent manner. Acts as inhibitor of monocyte chemotaxis. Interacts with SLIT1 and SLIT2 in a glycosylation- dependent manner. By high glucose through TGFB1-mediated pathways in mesangial cell. Down-regulated in tumor cell lines. Highly expressed in small intestine, fetal brain and colon. Weakly expressed in brain, ovary, prostate, pancreas and skeletal muscle. In brain found in the region localized around the internal capsule in the large subcortical nuclei, including caudate, putamen, substantia nigra, thalamus and subthalamus. Predominantly expressed in normal cells including neurons, astrocytes and fibroblasts. Belongs to the DAN family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Secreted; Secreted, signal peptide. Cellular Component: extracellular space; cell surface; extracellular region. Molecular Function: heparan sulfate proteoglycan binding; transmembrane receptor protein tyrosine kinase activator activity; vascular endothelial growth factor receptor 2 binding; cytokine activity; receptor agonist activity. Biological Process: limb development; collagen fibril organization; transmembrane receptor protein tyrosine kinase activation (dimerization); positive regulation of transcription, DNA-dependent; negative regulation of chondrocyte differentiation; positive regulation of receptor internalization; cell morphogenesis; positive regulation of NF-kappaB import into nucleus; signal transduction; negative regulation of BMP signaling pathway; activation of NF-kappaB transcription factor; negative regulation of bone mineralization; cell-cell signaling; positive regulation of cell proliferation; negative regulation of leukocyte chemotaxis; angiogenesis; proximal/distal pattern formation; embryonic limb morphogenesis; determination of dorsal identity; positive regulation of telomerase activity; regulation of stress-activated MAPK cascade; positive regulation of angiogenesis; organ morphogenesis; cell migration during sprouting angiogenesis; ureteric bud branching; regulation of focal adhesion formation; negative regulation of bone remodeling; endothelial cell migration; negative regulation of osteoblast proliferation; positive regulation of transcription from RNA polymerase II promoter; negative regulation of cell growth; negative regulation of transcription, DNA-dependent; negative regulation of apoptosis

24-Strip-Wells

270 USD

48-Strip-Wells

495

96-Strip-Wells

665

5x96-Strip-Wells

2715

10x96-Strip-Wells

4895