ELISA/assay

Ephrin B1 (EFNB1) ELISA Kit

MBS2020110

96 Strip Wells

780 USD

ELISA Kit

Ephrin B1 (EFNB1) ELISA Kit

Ephrin B1 (EFNB1)

CFND; CFNS; EFL3; EPLG2; Elk-L; LERK2; EPH-related receptor tyrosine kinase ligand 2; Craniofrontonasal Syndrome(Craniofrontonasal Dysplasia)

ephrin-B1; Ephrin-B1; ephrin-B1; ephrin-B1; EFL-3; ELK ligand; ELK-L; EPH-related receptor tyrosine kinase ligand 2; LERK-2

EFNB1

EFNB1; EFNB1; CFND; CFNS; EFB1; EFL3; EPLG2; Elk-L; LERK2; EFL3; EPLG2; LERK2; ELK-L; LERK-2

EFNB1_HUMAN

Human

346

This assay has high sensitivity and excellent specificity for detection of Ephrin B1 (EFNB1). No significant cross-reactivity or interference between Ephrin B1 (EFNB1) and analogues was observed.

For unopened kit: All the reagents should be kept according to the labels on vials. The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 degree C upon receipt while the others should be at 4 degree C. For opened kit: When the kit is opened, the remaining reagents still need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and reseal along entire edge of zip-seal. The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Sample Types: Tissue homogenates, cell lysates and other biological fluids. Detection Range: 0.156-10ng/mL. Sensitivity: The minimum detectable dose of this kit is typically less than 0.067ng/mL.

Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Ephrin B1 (EFNB1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Ephrin B1 (EFNB1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 . Intra-Assay: CV<10% . Inter-Assay: CV<12% . Assay Procedure Summary: 1. Prepare all reagents, samples and standards;. 2. Add 100uL standard or sample to each well. Incubate 2 hours at 37 degree C;. 3. Aspirate and add 100uL prepared Detection Reagent A. Incubate 1 hour at 37 degree C;. 4. Aspirate and wash 3 times;. 5. Add 100uL prepared Detection Reagent B. Incubate 30 minutes at 37 degree C;. 6. Aspirate and wash 5 times;. 7. Add 90uL Substrate Solution. Incubate 15-25 minutes at 37 degree C;. 8. Add 50uL Stop Solution. Read at 450nm immediately.

Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Ephrin B1 (EFNB1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Ephrin B1 (EFNB1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Ephrin B1 (EFNB1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Ephrin B1 (EFNB1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

4758248

NP_004420.1

NM_004429.4

P98172

38,007 Da

Axon Guidance Pathway 83065!!Axon Guidance Pathway 476!!Axon Guidance Pathway 105688!!Developmental Biology Pathway 477129!!EPH-Ephrin Signaling Pathway 1127691!!EPH-ephrin Mediated Repulsion Of Cells Pathway 1127695!!EPHB Forward Signaling Pathway 138047!!EPHB-mediated Forward Signaling Pathway 1127693!!Ephrin B Reverse Signaling Pathway 138052!!Ephrin Signaling Pathway 1127694

The protein encoded by this gene is a type I membrane protein and a ligand of Eph-related receptor tyrosine kinases. It may play a role in cell adhesion and function in the development or maintenance of the nervous system. [provided by RefSeq, Jul 2008]

96 Strip Wells

780 USD