ELISA/assay

Transforming Growth Factor Beta Induced Protein (TGFbI) ELISA Kit

MBS2020106

48-Strip-Wells

515 USD

ELISA Kit

Transforming Growth Factor Beta Induced Protein (TGFbI) ELISA Kit

Transforming Growth Factor Beta Induced Protein (TGFbI)

TGF-BI; CSD3; LCD1; CSD1; CSD2; BIGH3; CDB1; CDGG1; RGD-CAP; Kerato-Epithelin; RGD-Containing Collagen-Associated Protein

transforming growth factor induced protein; Transforming growth factor-beta-induced protein ig-h3; transforming growth factor-beta-induced protein ig-h3; transforming growth factor, beta-induced, 68kDa; Kerato-epithelin; RGD-containing collagen-associated protein; RGD-CAP

TGFbI

TGFBI; TGFBI; CSD; CDB1; CDG2; CSD1; CSD2; CSD3; EBMD; LCD1; BIGH3; CDGG1; BIGH3; Beta ig-h3; RGD-CAP

BGH3_HUMAN

Human

683

This assay has high sensitivity and excellent specificity for detection of Transforming Growth Factor Beta Induced Protein (TGFbI). No significant cross-reactivity or interference between Transforming Growth Factor Beta Induced Protein (TGFbI) and analogues was observed.

For unopened kit: All the reagents should be kept according to the labels on vials. The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 degree C upon receipt while the others should be at 4 degree C. For opened kit: When the kit is opened, the remaining reagents still need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and reseal along entire edge of zip-seal. The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Samples: Serum, plasma, tissue homogenates and other biological fluids. Assay Type: Sandwich. Detection Range: 0.625-40ng/mL. Sensitivity: Typically less than 0.237ng/mL.

Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Transforming Growth Factor Beta Induced Protein (TGFbI) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Transforming Growth Factor Beta Induced Protein (TGFbI) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 . Intra-Assay: CV<10% . Inter-Assay: CV<12% . Assay Procedure Summary: 1. Prepare all reagents, samples and standards;. 2. Add 100uL standard or sample to each well. Incubate 2 hours at 37 degree C;. 3. Aspirate and add 100uL prepared Detection Reagent A. Incubate 1 hour at 37 degree C;. 4. Aspirate and wash 3 times;. 5. Add 100uL prepared Detection Reagent B. Incubate 30 minutes at 37 degree C;. 6. Aspirate and wash 5 times;. 7. Add 90uL Substrate Solution. Incubate 15-25 minutes at 37 degree C;. 8. Add 50uL Stop Solution. Read at 450nm immediately.

Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Transforming Growth Factor Beta Induced Protein (TGFbI). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Transforming Growth Factor Beta Induced Protein (TGFbI). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Transforming Growth Factor Beta Induced Protein (TGFbI), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Transforming Growth Factor Beta Induced Protein (TGFbI) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

339568

AAA61163.1

Q15582

74,681 Da

Amyloids Pathway (366238); Disease Pathway (530764)

This gene encodes an RGD-containing protein that binds to type I, II and IV collagens. The RGD motif is found in many extracellular matrix proteins modulating cell adhesion and serves as a ligand recognition sequence for several integrins. This protein plays a role in cell-collagen interactions and may be involved in endochondrial bone formation in cartilage. The protein is induced by transforming growth factor-beta and acts to inhibit cell adhesion. Mutations in this gene are associated with multiple types of corneal dystrophy. [provided by RefSeq, Jul 2008]

betaIG-H3: Binds to type I, II, and IV collagens. This adhesion protein may play an important role in cell-collagen interactions. In cartilage, may be involved in endochondral bone formation. By TGFB1. Highly expressed in the corneal epithelium. Protein type: Secreted; Cell adhesion; Secreted, signal peptide; Extracellular matrix. Chromosomal Location of Human Ortholog: 5q31. Cellular Component: extracellular matrix; proteinaceous extracellular matrix; extracellular space; extracellular region; plasma membrane; basement membrane; trans-Golgi network. Molecular Function: collagen binding; integrin binding; protein binding; extracellular matrix binding. Biological Process: cell proliferation; extracellular matrix organization and biogenesis; visual perception; response to stimulus; chondrocyte differentiation; angiogenesis; negative regulation of cell adhesion; cell adhesion. Disease: Corneal Dystrophy, Epithelial Basement Membrane; Corneal Dystrophy, Lattice Type Iiia; Corneal Dystrophy, Avellino Type; Corneal Dystrophy Of Bowman Layer, Type Ii; Corneal Dystrophy, Lattice Type I; Corneal Dystrophy Of Bowman Layer, Type I; Corneal Dystrophy, Groenouw Type I

48-Strip-Wells

515 USD

96-Strip-Wells

695

5x96-Strip-Wells

2685

10x96-Strip-Wells

4850