Cystic Fibrosis Transmembrane Conductance Regulator (CFTR) ELISA Kit | MyBioSource MBS2020064 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Cystic Fibrosis Transmembrane Conductance Regulator (CFTR) ELISA Kit

catalog :

MBS2020064

quantity :

24-Strip-Wells

price :

270 USD

more info or order :

MyBioSource product webpage

image

image 1 :

product information

catalog number :

MBS2020064

products type :

ELISA Kit

products full name :

Cystic Fibrosis Transmembrane Conductance Regulator (CFTR) ELISA Kit

products short name :

[Cystic Fibrosis Transmembrane Conductance Regulator (CFTR)]

products name syn :

[ABC35; ABCC7; CF; CFTR/MRP; MRP7; TNR-CFTR; ATP-Binding Cassette Subfamily C,Member 7; Channel conductance-controlling ATPase; cAMP-dependent chloride channel]

other names :

[cystic fibrosis transmembrane conductance regulator; Cystic fibrosis transmembrane conductance regulator; cystic fibrosis transmembrane conductance regulator; cystic fibrosis transmembrane conductance regulator (ATP-binding cassette sub-family C, member 7); ATP-binding cassette sub-family C member 7; Channel conductance-controlling ATPase (EC:3.6.3.49); cAMP-dependent chloride channel]

products gene name :

[CFTR]

other gene names :

[CFTR; CFTR; CF; MRP7; ABC35; ABCC7; CFTR/MRP; TNR-CFTR; dJ760C5.1; ABCC7; CFTR]

uniprot entry name :

CFTR_HUMAN

reactivity :

Human

sequence length :

1480

specificity :

This assay has high sensitivity and excellent specificity for detection of CES2. No significant cross-reactivity or interference between CES2 and analogues was observed.

storage stability :

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

image1 heading :

Typical Testing Data/Standard Curve (for reference only)

other info1 :

Samples: Human Serum, Plasma, Tissue Homogenates And Other Biological Fluids. Assay Type: Quantitative Sandwich. Detection Range: 0.625-40ng/mL. Sensitivity: 0.245ng/mL.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level CES2 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level CES2 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%

products categories :

Immune molecule

products description :

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of CES2 in human serum, plasma, tissue homogenates and other biological fluids. Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to CES2. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to CES2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain CES2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of CES2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

ncbi gi num :

90421313

ncbi acc num :

NP_000483.3

ncbi gb acc num :

NM_000492.3

uniprot acc num :

P13569

ncbi mol weight :

69,230 Da

ncbi pathways :

ABC Transporters Pathway (83035); ABC Transporters Pathway (436); ABC-family Proteins Mediated Transport Pathway (106573); AMPK Signaling Pathway (989139); AMPK Signaling Pathway (992181); Bile Secretion Pathway (193146); Bile Secretion Pathway (193095); Gastric Acid Secretion Pathway (154409); Gastric Acid Secretion Pathway (154383); Pancreatic Secretion Pathway (169306)

ncbi summary :

This gene encodes a member of the ATP-binding cassette (ABC) transporter superfamily. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MRP subfamily that is involved in multi-drug resistance. The encoded protein functions as a chloride channel and controls the regulation of other transport pathways. Mutations in this gene are associated with the autosomal recessive disorders cystic fibrosis and congenital bilateral aplasia of the vas deferens. Alternatively spliced transcript variants have been described, many of which result from mutations in this gene. [provided by RefSeq, Jul 2008]

uniprot summary :

CFTR: a member of the MRP subfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. Functions as a chloride channel and controls the regulation of other transport pathways. Mutations have been observed in patients with the autosomal recessive disorders cystic fibrosis (CF) and congenital bilateral aplasia of the vas deferens (CBAVD). Alternative splice. Protein type: Membrane protein, multi-pass; Channel, chloride; Transporter, ABC family; Membrane protein, integral; Transporter; Hydrolase; EC 3.6.3.49. Chromosomal Location of Human Ortholog: 7q31.2. Cellular Component: recycling endosome; cell surface; cytoplasmic vesicle membrane; microvillus; protein complex; early endosome membrane; basolateral plasma membrane; apical plasma membrane; early endosome; cytoplasm; plasma membrane. Molecular Function: bicarbonate transmembrane transporter activity; protein binding; chloride channel activity; enzyme binding; chloride channel inhibitor activity; chloride transmembrane transporter activity; channel-conductance-controlling ATPase activity; ATP-binding and phosphorylation-dependent chloride channel activity; ATP binding; PDZ domain binding. Biological Process: intracellular pH elevation; response to drug; response to peptide hormone stimulus; cholesterol transport; iodide transport; transepithelial chloride transport; water transport; membrane hyperpolarization; respiratory gaseous exchange; positive regulation of vasodilation; cholesterol biosynthetic process; vasodilation; cellular response to hormone stimulus; response to estrogen stimulus; bicarbonate transport; transport; response to cytokine stimulus; transmembrane transport; sperm capacitation; lung development. Disease: Vas Deferens, Congenital Bilateral Aplasia Of; Bronchiectasis With Or Without Elevated Sweat Chloride 1; Cystic Fibrosis; Pancreatitis, Hereditary

size1 :

24-Strip-Wells

price1 :

270 USD

size2 :

48-Strip-Wells

price2 :

495

size3 :

96-Strip-Wells

price3 :

665

size4 :

5x96-Strip-Wells

price4 :

2715

size5 :

10x96-Strip-Wells

price5 :

4895

more info or order :

MyBioSource product webpage