Bone Morphogenetic Protein 1 (BMP1) ELISA Kit | MyBioSource MBS2020062 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Bone Morphogenetic Protein 1 (BMP1) ELISA Kit

catalog :

MBS2020062

quantity :

24-Strip-Wells

price :

255 USD

product information

catalog number :

MBS2020062

products type :

ELISA Kit

products full name :

Bone Morphogenetic Protein 1 (BMP1) ELISA Kit

products short name :

[Bone Morphogenetic Protein 1 (BMP1)]

products name syn :

[PCOLC; PCP; TLD; mTld; Procollagen C Proteinase; Mammalian tolloid protein]

other names :

[bone morphogenetic protein 1; Bone morphogenetic protein 1; bone morphogenetic protein 1; bone morphogenetic protein 1; Mammalian tolloid protein; mTld; Procollagen C-proteinase; PCP]

products gene name :

[BMP1]

other gene names :

[Bmp1; Bmp1; Pcp; Tld; BMP-1; mTld; PCP]

uniprot entry name :

BMP1_MOUSE

reactivity :

Human

sequence length :

991

specificity :

This assay has high sensitivity and excellent specificity for detection of Bone Morphogenetic Protein 1 (BMP1). No significant cross-reactivity or interference between Bone Morphogenetic Protein 1 (BMP1) and analogues was observed.

storage stability :

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

image1 heading :

Typical Testing Data/Standard Curve (for reference only)

other info1 :

Assay Type: Double-antibody Sandwich. Samples: Serum, Plasma, Tissue homogenates and Other Biological Fluids. Detection Range: 15.6-1,000pg/mL. Sensitivity: < 6.0pg/mL

other info2 :

Application: Enzyme-linked immunosorbent assay for Antigen Detection. Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Bone Morphogenetic Protein 1 (BMP1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Bone Morphogenetic Protein 1 (BMP1) were tested on 3 different plates, 8 replicates in each plate. CV(%) =: SD/meanX100. Intra-Assay: CV<10%. Inter-Assay: CV<12%

products categories :

Developmental science

products description :

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Bone Morphogenetic Protein 1 (BMP1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Bone Morphogenetic Protein 1 (BMP1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Bone Morphogenetic Protein 1 (BMP1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Bone Morphogenetic Protein 1 (BMP1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

ncbi gi num :

42734447

ncbi acc num :

NP_033885.2

ncbi gb acc num :

NM_009755.3

uniprot acc num :

P98063

ncbi mol weight :

111,666 Da

ncbi pathways :

Adipogenesis Pathway (198299); Anchoring Fibril Formation Pathway (1110306); Assembly Of Collagen Fibrils And Other Multimeric Structures Pathway (1110305); Collagen Biosynthesis And Modifying Enzymes Pathway (1110304); Collagen Formation Pathway (1110303); Crosslinking Of Collagen Fibrils Pathway (1110307); Degradation Of The Extracellular Matrix Pathway (1110315); Extracellular Matrix Organization Pathway (1110302); HDL-mediated Lipid Transport Pathway (1110839); Lipid Digestion, Mobilization, And Transport Pathway (1110834)

ncbi summary :

This gene encodes a metalloproteinase that plays an essential role in the formation of the extracellular matrix and is also able to induce ectopic bone formation. Unlike other bone morphogenetic proteins, the protein encoded by this gene is not closely related to transforming growth factor-beta. This protein plays in role several developmental processes. In humans, mutations in this gene are associated with osteogenesis imperfecta and with increased bone mineral density and multiple recurrent fractures. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2013]

uniprot summary :

BMP1: Cleaves the C-terminal propeptides of procollagen I, II and III. Induces cartilage and bone formation. May participate in dorsoventral patterning during early development by cleaving chordin (CHRD). Defects in BMP1 are a cause of autosomal recessive osteogenesis imperfecta (AR-OI). A connective tissue disorder characterized by bone fragility, progressively deforming bones, bowing of limbs due to multiple fractures, very short stature, a triangular face, severe scoliosis, and grayish sclera. AR-OI due to BMP1 mutations belongs to the group of osteogenesis imperfecta type III in the Sillence classification. Belongs to the peptidase M12A family. 7 isoforms of the human protein are produced by alternative splicing. Protein type: Protease; Cytokine; EC 3.4.24.19. Cellular Component: Golgi apparatus; extracellular space; proteinaceous extracellular matrix; extracellular region. Molecular Function: peptidase activity; protein binding; growth factor activity; hydrolase activity; zinc ion binding; metallopeptidase activity; metalloendopeptidase activity; metal ion binding; cytokine activity; calcium ion binding. Biological Process: ossification; multicellular organismal development; cartilage development; cell differentiation; proteolysis

size1 :

24-Strip-Wells

price1 :

255 USD

size2 :

48-Strip-Wells

price2 :

455

size3 :

96-Strip-Wells

price3 :

610

size4 :

5x96-Strip-Wells

price4 :

2470

size5 :

10x96-Strip-Wells

price5 :

4445