ELISA/assay

Excitatory Amino Acid Transporter 2 (EAAT2) ELISA Kit

MBS2020019

48-Strip-Wells

605 USD

ELISA Kit

Excitatory Amino Acid Transporter 2 (EAAT2) ELISA Kit

Excitatory Amino Acid Transporter 2 (EAAT2)

SLC1A2; EAAT2; GLT-1; Solute Carrier Family 1 Member 2; Glutamate/aspartate transporter II; Sodium-dependent glutamate/aspartate transporter 2

excitatory amino acid transporter 2 isoform 2; Excitatory amino acid transporter 2; excitatory amino acid transporter 2; solute carrier family 1 (glial high affinity glutamate transporter), member 2; Glutamate/aspartate transporter II; Sodium-dependent glutamate/aspartate transporter 2; Solute carrier family 1 member 2

EAAT2

SLC1A2; SLC1A2; HBGT; EAAT2; GLT-1; EAAT2; GLT1

EAA2_HUMAN

Rat

565

This assay has high sensitivity and excellent specificity for detection of EAAT2. No significant cross-reactivity or interference between EAAT2 and analogues was observed.

For unopened kit: All the reagents should be kept according to the labels on vials. The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 degree C upon receipt while the others should be at 4 degree C. For opened kit: When the kit is opened, the remaining reagents still need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and reseal along entire edge of zip-seal. The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Samples: Rat tissue homogenates, cell lysates and other biological fluids. Assay Type: Sandwich. Detection Range: 0.156-10ng/mL. The standard curve concentrations used for the ELISA's were 10ng/mL, 5ng/mL, 2.5ng/mL, 1.25ng/mL, 0.625ng/mL, 0.312ng/mL, 0.156ng/mL. Sensitivity: 0.057ng/mL.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level EAAT2 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level EAAT2 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of EAAT2 in rat tissue homogenates, cell lysates and other biological fluids. Principle of the Assay The microtiter plate provided in this kit has been pre-coated with an antibody specific to EAAT2. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to EAAT2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain EAAT2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of EAAT2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

307746865

NP_001182657.1

NM_001195728.2

61,008 Da

Amyotrophic Lateral Sclerosis (ALS) Pathway (83099); Amyotrophic Lateral Sclerosis (ALS) Pathway (511); Astrocytic Glutamate-Glutamine Uptake And Metabolism Pathway (106533); Glutamatergic Synapse Pathway (213818); Glutamatergic Synapse Pathway (213811); Neuronal System Pathway (106513); Neurotransmitter Uptake And Metabolism In Glial Cells Pathway (106532); SLC-mediated Transmembrane Transport Pathway (119558); Transmembrane Transport Of Small Molecules Pathway (106572); Transmission Across Chemical Synapses Pathway (106516)

This gene encodes a member of a family of solute transporter proteins. The membrane-bound protein is the principal transporter that clears the excitatory neurotransmitter glutamate from the extracellular space at synapses in the central nervous system. Glutamate clearance is necessary for proper synaptic activation and to prevent neuronal damage from excessive activation of glutamate receptors. Mutations in and decreased expression of this protein are associated with amyotrophic lateral sclerosis. Alternatively spliced transcript variants of this gene have been identified. [provided by RefSeq, Sep 2010]

GLT1: an integral membrane protein belonging to the sodium:dicarboxylate (SDF) symporter family. Transports L-glutamate and also L- and D-aspartate. Essential for terminating the postsynaptic action of glutamate by rapidly removing released glutamate from the synaptic cleft. Acts as a symport by co-transporting sodium. Protein type: Transporter; Transporter, SLC family; Membrane protein, multi-pass; Membrane protein, integral. Chromosomal Location of Human Ortholog: 11p13-p12. Cellular Component: membrane; plasma membrane; integral to membrane; axolemma. Molecular Function: protein binding; glutamate:sodium symporter activity; sodium:dicarboxylate symporter activity; L-glutamate transmembrane transporter activity. Biological Process: response to drug; synaptic transmission; positive regulation of glucose import; L-glutamate import; telencephalon development; adult behavior; multicellular organism growth; cellular response to extracellular stimulus; response to wounding; multicellular organismal aging; ion transport; response to amino acid stimulus; transmembrane transport; visual behavior

48-Strip-Wells

605 USD

96-Strip-Wells

820

5x96-Strip-Wells

3220

10x96-Strip-Wells

5795