ELISA/assay

ELISA Kit for Secreted Frizzled Related Protein 2 (SFRP2)

MBS2019998

48-Strip-Wells

575 USD

ELISA Kit

ELISA Kit for Secreted Frizzled Related Protein 2 (SFRP2)

Secreted Frizzled Related Protein 2 (SFRP2)

secreted frizzled-related protein 2; Secreted frizzled-related protein 2; secreted frizzled-related protein 2; SARP-1; sFRP-2; secreted apoptosis related protein 1; secreted apoptosis-related protein 1; secreted frizzled-related protein 2; Secreted apoptosis-related protein 1; SARP-1

SFRP2

SFRP2; SFRP2; FRP-2; SARP1; SDF-5; FRP2; SARP1; FRP-2; sFRP-2; SARP-1

SFRP2_HUMAN

Mouse

295

This assay has high sensitivity and excellent specificity for detection of SFRP2. No significant cross-reactivity or interference between SFRP2 and analogues was observed.

For unopened kit, all the reagents should be kept according to the label on each vial. The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 degree C while others should be at 4 degree C.

Samples: Mouse serum, plasma, tissue homogenates and other biological fluids. Assay Type: Sandwich. Detection Range: 125-8,000pg/mL. The standard curve concentrations used for the ELISA's were 8,000pg/mL, 4,000pg/mL, 2,000pg/mL, 1,000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL. Sensitivity: The minimum detectable dose of SFRP2 is typically less than 46pg/mL. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined by adding two standard deviations to the mean optical density value of twenty zero standard replicates and calculating the corresponding concentration.

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of SFRP2 in mouse serum, plasma, tissue homogenates and other biological fluids. Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level SFRP2 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level SFRP2 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to SFRP2. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to SFRP2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain SFRP2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of SFRP2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

48475052

NP_003004.1

NM_003013.2

Q96HF1

33,490 Da

Disease Pathway (530764); RNF Mutants Show Enhanced WNT Signaling And Proliferation Pathway (1127632); Signal Transduction Pathway (477114); Signaling By WNT In Cancer Pathway (1127614); Signaling By Wnt Pathway (106510); TCF Dependent Signaling In Response To WNT Pathway (1127516); Wnt Signaling Pathway NetPath (198799); Wnt Signaling Pathway (83061); Wnt Signaling Pathway (471); XAV939 Inhibits Tankyrase, Stabilizing AXIN Pathway (1127633)

This gene encodes a member of the SFRP family that contains a cysteine-rich domain homologous to the putative Wnt-binding site of Frizzled proteins. SFRPs act as soluble modulators of Wnt signaling. Methylation of this gene is a potential marker for the presence of colorectal cancer. [provided by RefSeq, Jul 2008]

SFRP2: Soluble frizzled-related proteins (sFRPS) function as modulators of Wnt signaling through direct interaction with Wnts. They have a role in regulating cell growth and differentiation in specific cell types. SFRP2 may be important for eye retinal development and for myogenesis. Belongs to the secreted frizzled-related protein (sFRP) family. Protein type: Secreted; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 4q31.3. Cellular Component: extracellular matrix; extracellular space; integral to membrane. Molecular Function: integrin binding; G-protein coupled receptor activity; Wnt-protein binding; Wnt receptor activity; fibronectin binding; receptor agonist activity. Biological Process: convergent extension involved in axis elongation; collagen fibril organization; negative regulation of Wnt receptor signaling pathway; positive regulation of apoptosis; negative regulation of caspase activity; negative regulation of BMP signaling pathway; negative regulation of cell proliferation; cell-cell signaling; negative regulation of mesodermal cell fate specification; positive regulation of cell proliferation; negative regulation of cell migration; response to nutrient; negative regulation of epithelial cell proliferation; response to drug; negative regulation of JNK activity; negative regulation of peptidyl-tyrosine phosphorylation; male gonad development; positive regulation of cell adhesion mediated by integrin; digestive tract morphogenesis; chondrocyte development; positive regulation of cell growth; positive regulation of peptidyl-serine phosphorylation; patterning of blood vessels; positive regulation of osteoblast differentiation; positive regulation of angiogenesis; neural tube closure; cellular response to extracellular stimulus; positive regulation of fat cell differentiation; positive regulation of transcription from RNA polymerase II promoter; embryonic digit morphogenesis; negative regulation of cell growth; negative regulation of transcription, DNA-dependent

48-Strip-Wells

575 USD

96-Strip-Wells

785

5x96-Strip-Wells

3055

10x96-Strip-Wells

5495