Carboxypeptidase A3, Mast Cell (CPA3) ELISA Kit | MyBioSource MBS2019985 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Carboxypeptidase A3, Mast Cell (CPA3) ELISA Kit

catalog :

MBS2019985

quantity :

48-Strip-Wells

price :

565 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS2019985

products type :

ELISA Kit

products full name :

Carboxypeptidase A3, Mast Cell (CPA3) ELISA Kit

products short name :

Carboxypeptidase A3, Mast Cell (CPA3)

products name syn :

MC-CPA; Mast cell carboxypeptidase A

other names :

carboxypeptidase A3 (mast cell); Mast cell carboxypeptidase A; mast cell carboxypeptidase A; carboxypeptidase A3 (mast cell); Carboxypeptidase A3

products gene name :

CPA3

other gene names :

CPA3; CPA3; MC-CPA; MC-CPA

uniprot entry name :

CBPA3_HUMAN

reactivity :

Human

sequence length :

417

specificity :

This assay has high sensitivity and excellent specificity for detection of Carboxypeptidase A3, Mast Cell (CPA3). No significant cross-reactivity or interference between Carboxypeptidase A3, Mast Cell (CPA3) and analogues was observed.

storage stability :

For unopened kit: All the reagents should be kept according to the labels on vials. The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 degree C upon receipt while the others should be at 4 degree C. For opened kit: When the kit is opened, the remaining reagents still need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and reseal along entire edge of zip-seal. The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

other info1 :

Samples: Serum, plasma and other biological fluids. Assay Type: Sandwich. Detection Range: 7.81-500pg/mL. Sensitivity: Typically less than 3.2pg/mL.

other info2 :

Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Carboxypeptidase A3, Mast Cell (CPA3) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Carboxypeptidase A3, Mast Cell (CPA3) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 . Intra-Assay: CV<10% . Inter-Assay: CV<12% . Assay Procedure Summary: 1. Prepare all reagents, samples and standards;. 2. Add 100uL standard or sample to each well. Incubate 2 hours at 37 degree C;. 3. Aspirate and add 100uL prepared Detection Reagent A. Incubate 1 hour at 37 degree C;. 4. Aspirate and wash 3 times;. 5. Add 100uL prepared Detection Reagent B. Incubate 30 minutes at 37 degree C;. 6. Aspirate and wash 5 times;. 7. Add 90uL Substrate Solution. Incubate 15-25 minutes at 37 degree C;. 8. Add 50uL Stop Solution. Read at 450nm immediately.

products description :

Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Carboxypeptidase A3, Mast Cell (CPA3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Carboxypeptidase A3, Mast Cell (CPA3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Carboxypeptidase A3, Mast Cell (CPA3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Carboxypeptidase A3, Mast Cell (CPA3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

ncbi gi num :

119599308

ncbi acc num :

EAW78902.1

uniprot acc num :

P15088

ncbi mol weight :

48,670 Da

ncbi pathways :

Metabolism Of Angiotensinogen To Angiotensins Pathway (685555); Metabolism Of Proteins Pathway (106230); Pancreatic Secretion Pathway (169306); Pancreatic Secretion Pathway (169295); Peptide Hormone Metabolism Pathway (771603); Protein Digestion And Absorption Pathway (172847); Protein Digestion And Absorption Pathway (171868); Renin-angiotensin System Pathway (83075); Renin-angiotensin System Pathway (486)

ncbi summary :

Three different forms of human pancreatic procarboxypeptidase A have been isolated. This gene encodes a form which is obtained as a binary complex of a procarboxypeptidase A with proproteinase E and functions as a secretory granule metalloexopeptidase. [provided by RefSeq, Jan 2009]

uniprot summary :

CPA3: Three different forms of human pancreatic procarboxypeptidase A have been isolated. This gene encodes a form which is obtained as a binary complex of a procarboxypeptidase A with proproteinase E and functions as a secretory granule metalloexopeptidase. [provided by RefSeq, Jan 2009]. Protein type: Protease; EC 3.4.17.1. Chromosomal Location of Human Ortholog: 3q24. Cellular Component: transport vesicle; extracellular region; secretory granule. Molecular Function: zinc ion binding; metallocarboxypeptidase activity. Biological Process: cellular protein metabolic process; angiotensin maturation; proteolysis

size1 :

48-Strip-Wells

price1 :

565 USD

size2 :

96-Strip-Wells

price2 :

765

size3 :

5x96-Strip-Wells

price3 :

2975

size4 :

10x96-Strip-Wells

price4 :

5340

more info or order :

MyBioSource product webpage