Carbonic Anhydrase VI (CA6) ELISA Kit | MyBioSource MBS2019945 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Carbonic Anhydrase VI (CA6) ELISA Kit

catalog :

MBS2019945

quantity :

48-Strip-Wells

price :

565 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS2019945

products type :

ELISA Kit

products full name :

Carbonic Anhydrase VI (CA6) ELISA Kit

products short name :

Carbonic Anhydrase VI (CA6)

products name syn :

CA-VI; CAVI; GUSTIN; Carbonate dehydratase VI; Salivary carbonic anhydrase; Secreted carbonic anhydrase

other names :

carbonic anhydrase VI; Carbonic anhydrase 6; carbonic anhydrase 6; carbonic anhydrase VI; Carbonate dehydratase VI; Carbonic anhydrase VI; CA-VI; Salivary carbonic anhydrase; Secreted carbonic anhydrase

products gene name :

CA6

other gene names :

CA6; CA6; CA-VI; GUSTIN; CA-VI

uniprot entry name :

CAH6_HUMAN

reactivity :

Human

sequence length :

308

specificity :

This assay has high sensitivity and excellent specificity for detection of Carbonic Anhydrase VI (CA6). No significant cross-reactivity or interference between Carbonic Anhydrase VI (CA6) and analogues was observed.

storage stability :

For unopened kit: All the reagents should be kept according to the labels on vials. The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 degree C upon receipt while the others should be at 4 degree C. For opened kit: When the kit is opened, the remaining reagents still need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and reseal along entire edge of zip-seal. The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

other info1 :

Samples: Serum, plasma, saliva and other biological fluids. Assay Type: Sandwich. Detection Range: 15.63-1000pg/mL. Sensitivity: Typically less than 6.8pg/mL.

other info2 :

Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Carbonic Anhydrase VI (CA6) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Carbonic Anhydrase VI (CA6) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 . Intra-Assay: CV<10% . Inter-Assay: CV<12% . Assay Procedure Summary: 1. Prepare all reagents, samples and standards;. 2. Add 100uL standard or sample to each well. Incubate 2 hours at 37 degree C;. 3. Aspirate and add 100uL prepared Detection Reagent A. Incubate 1 hour at 37 degree C;. 4. Aspirate and wash 3 times;. 5. Add 100uL prepared Detection Reagent B. Incubate 30 minutes at 37 degree C;. 6. Aspirate and wash 5 times;. 7. Add 90uL Substrate Solution. Incubate 15-25 minutes at 37 degree C;. 8. Add 50uL Stop Solution. Read at 450nm immediately.

products description :

Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Carbonic Anhydrase VI (CA6). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Carbonic Anhydrase VI (CA6). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Carbonic Anhydrase VI (CA6), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Carbonic Anhydrase VI (CA6) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

ncbi gi num :

119592012

ncbi acc num :

EAW71606.1

uniprot acc num :

P23280

ncbi mol weight :

28,687 Da

ncbi pathways :

Metabolism Pathway (477135); Nitrogen Metabolism Pathway (83025); Nitrogen Metabolism Pathway (416); Reversible Hydration Of Carbon Dioxide Pathway (645352)

ncbi summary :

The protein encoded by this gene is one of several isozymes of carbonic anhydrase. This protein is found only in salivary glands and saliva and protein may play a role in the reversible hydratation of carbon dioxide though its function in saliva is unknown. [provided by RefSeq, Jul 2008]

uniprot summary :

CA6: Reversible hydration of carbon dioxide. Its role in saliva is unknown. Belongs to the alpha-carbonic anhydrase family. Protein type: Secreted; Lyase; EC 4.2.1.1; Energy Metabolism - nitrogen; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 1p36.2. Cellular Component: extracellular space; cytoplasm; extracellular region. Molecular Function: carbonate dehydratase activity; zinc ion binding. Biological Process: bicarbonate transport; one-carbon compound metabolic process; detection of chemical stimulus involved in sensory perception of bitter taste

size1 :

48-Strip-Wells

price1 :

565 USD

size2 :

96-Strip-Wells

price2 :

765

size3 :

5x96-Strip-Wells

price3 :

2975

size4 :

10x96-Strip-Wells

price4 :

5340

more info or order :

MyBioSource product webpage