Matrix Gla Protein (MGP) ELISA Kit | MyBioSource MBS2019935 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Matrix Gla Protein (MGP) ELISA Kit

catalog :

MBS2019935

quantity :

48-Strip-Wells

price :

575 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS2019935

products type :

ELISA Kit

products full name :

Matrix Gla Protein (MGP) ELISA Kit

products short name :

Matrix Gla Protein (MGP)

products name syn :

MGLAP; GIG36; Cell growth-inhibiting gene 36 protein

other names :

matrix Gla protein; Matrix Gla protein; matrix Gla protein; matrix Gla protein; Cell growth-inhibiting gene 36 protein

products gene name :

MGP

other gene names :

MGP; MGP; NTI; GIG36; MGLAP; MGLAP; MGP

uniprot entry name :

MGP_HUMAN

reactivity :

Rat

sequence length :

103

specificity :

This assay has high sensitivity and excellent specificity for detection of Matrix Gla Protein (MGP). No significant cross-reactivity or interference between Matrix Gla Protein (MGP) and analogues was observed.

storage stability :

For unopened kit: All the reagents should be kept according to the labels on vials. The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 degree C upon receipt while the others should be at 4 degree C. For opened kit: When the kit is opened, the remaining reagents still need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and reseal along entire edge of zip-seal. The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

other info1 :

Samples: Serum, plasma, tissue homogenates, Cell lysates, cell culture supernates and other biological fluids. Assay Type: Sandwich. Detection Range: 0.781-50ng/mL. Sensitivity: Typically less than 0.28ng/mL.

other info2 :

Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Matrix Gla Protein (MGP) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Matrix Gla Protein (MGP) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 . Intra-Assay: CV<10% . Inter-Assay: CV<12% . Assay Procedure Summary: 1. Prepare all reagents, samples and standards;. 2. Add 100uL standard or sample to each well. Incubate 2 hours at 37 degree C;. 3. Aspirate and add 100uL prepared Detection Reagent A. Incubate 1 hour at 37 degree C;. 4. Aspirate and wash 3 times;. 5. Add 100uL prepared Detection Reagent B. Incubate 30 minutes at 37 degree C;. 6. Aspirate and wash 5 times;. 7. Add 90uL Substrate Solution. Incubate 15-25 minutes at 37 degree C;. 8. Add 50uL Stop Solution. Read at 450nm immediately.

products description :

Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Matrix Gla Protein (MGP). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Matrix Gla Protein (MGP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Matrix Gla Protein (MGP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Matrix Gla Protein (MGP) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

ncbi gi num :

187591

ncbi acc num :

AAB53765.1

uniprot acc num :

P08493

ncbi mol weight :

15,325 Da

ncbi pathways :

Endochondral Ossification Pathway (198812); Validated Transcriptional Targets Of AP1 Family Members Fra1 And Fra2 Pathway (169349)

ncbi summary :

The protein encoded by this gene is secreted and likely acts as an inhibitor of bone formation. The encoded protein is found in the organic matrix of bone and cartilage. Defects in this gene are a cause of Keutel syndrome (KS). Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2010]

uniprot summary :

MGP: Associates with the organic matrix of bone and cartilage. Thought to act as an inhibitor of bone formation. Defects in MGP are the cause of Keutel syndrome (KS). KS is an autosomal recessive disorder characterized by abnormal cartilage calcification, peripheral pulmonary stenosis neural hearing loss and midfacial hypoplasia. Belongs to the osteocalcin/matrix Gla protein family. Protein type: Secreted, signal peptide; Secreted; Extracellular matrix. Chromosomal Location of Human Ortholog: 12p12.3. Cellular Component: extracellular matrix; proteinaceous extracellular matrix. Molecular Function: protein binding; structural constituent of bone; extracellular matrix structural constituent; calcium ion binding. Biological Process: ossification; cell differentiation; regulation of bone mineralization; cartilage condensation. Disease: Keutel Syndrome

size1 :

48-Strip-Wells

price1 :

575 USD

size2 :

96-Strip-Wells

price2 :

785

size3 :

5x96-Strip-Wells

price3 :

3065

size4 :

10x96-Strip-Wells

price4 :

5510

more info or order :

MyBioSource product webpage