Ghrelin-O-Acyltransferase (GOAT) ELISA Kit | MyBioSource MBS2019923 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Ghrelin-O-Acyltransferase (GOAT) ELISA Kit

catalog :

MBS2019923

quantity :

48-Strip-Wells

price :

610 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS2019923

products type :

ELISA Kit

products full name :

Ghrelin-O-Acyltransferase (GOAT) ELISA Kit

products short name :

Ghrelin-O-Acyltransferase (GOAT)

products name syn :

OACT4; MBOAT4; Membrane Bound O-Acyltransferase Domain Containing Protein 4

other names :

ghrelin O-acyltransferase; Ghrelin O-acyltransferase; ghrelin O-acyltransferase; membrane bound O-acyltransferase domain containing 4; Membrane-bound O-acyltransferase domain-containing protein 4; O-acyltransferase domain-containing protein 4

products gene name :

GOAT

other gene names :

MBOAT4; MBOAT4; GOAT; OACT4; FKSG89; GOAT; OACT4; O-acyltransferase domain-containing protein 4

uniprot entry name :

MBOA4_HUMAN

reactivity :

Human

sequence length :

435

specificity :

This assay has high sensitivity and excellent specificity for detection of GOAT. No significant cross-reactivity or interference between GOAT and analogues was observed.

storage stability :

For unopened kit: All the reagents should be kept according to the labels on vials. The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 degree C upon receipt while the others should be at 4 degree C. For opened kit: When the kit is opened, the remaining reagents still need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and reseal along entire edge of zip-seal. The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

other info1 :

Samples: Human serum, plasma, tissue homogenates and other biological fluids. Assay Type: Sandwich. Detection Range: 0.78-50ng/mL. The standard curve concentrations used for the ELISA's were 50ng/mL, 25ng/mL, 12.5ng/mL, 6.25ng/mL, 3.12ng/mL, 1.56ng/mL, 0.78ng/mL. Sensitivity: 0.29ng/mL.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level GOAT were tested 20 times on one plate, respectively. Intra-Assay: CV<10%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level GOAT were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%

products description :

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of GOAT in human serum, plasma, tissue homogenates and other biological fluids. Principle of the Assay The microtiter plate provided in this kit has been pre-coated with an antibody specific to GOAT. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to GOAT. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain GOAT, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of GOAT in the samples is then determined by comparing the O.D. of the samples to the standard curve.

ncbi gi num :

189571629

ncbi acc num :

NP_001094386.1

ncbi gb acc num :

NM_001100916.1

uniprot acc num :

Q96T53

ncbi mol weight :

37,721 Da

ncbi pathways :

Metabolism Of Proteins Pathway (106230); Peptide Hormone Metabolism Pathway (771603); Synthesis, Secretion, And Deacylation Of Ghrelin Pathway (119538)

uniprot summary :

MBOAT4: Mediates the octanoylation of ghrelin at Ser-3 . Can use a variety of fatty acids as substrates including octanoic acid, decanoic acid and tetradecanoic acid. Belongs to the membrane-bound acyltransferase family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Endoplasmic reticulum; Membrane protein, integral; EC 2.3.1.-; Transferase; Membrane protein, multi-pass. Chromosomal Location of Human Ortholog: 8p12. Cellular Component: endoplasmic reticulum membrane; integral to endoplasmic reticulum membrane. Molecular Function: serine O-acyltransferase activity. Biological Process: cellular protein metabolic process; peptidyl-serine octanoylation

size1 :

48-Strip-Wells

price1 :

610 USD

size2 :

96-Strip-Wells

price2 :

830

size3 :

5x96-Strip-Wells

price3 :

3265

size4 :

10x96-Strip-Wells

price4 :

5870

more info or order :

MyBioSource product webpage