ELISA/assay

Transient Receptor Potential Cation Channel Subfamily V, Member 1 (TRPV1) ELISA Kit

MBS2019906

24-Strip-Wells

270 USD

ELISA Kit

Transient Receptor Potential Cation Channel Subfamily V, Member 1 (TRPV1) ELISA Kit

[Transient Receptor Potential Cation Channel Subfamily V, Member 1 (TRPV1)]

[VR1; OTRPC1; Vanilloid Receptor Subtype 1; Capsaicin receptor; Osm-9-like TRP channel 1; Vanilloid receptor 1]

[transient receptor potential cation channel subfamily V member 1; Transient receptor potential cation channel subfamily V member 1; transient receptor potential cation channel subfamily V member 1; transient receptor potential cation channel, subfamily V, member 1; Capsaicin receptor; Osm-9-like TRP channel 1; OTRPC1; Vanilloid receptor 1]

[TRPV1]

[TRPV1; TRPV1; VR1; VR1; TrpV1; OTRPC1]

TRPV1_HUMAN

Human

839

This assay has high sensitivity and excellent specificity for detection of TRPV1. No significant cross-reactivity or interference between TRPV1 and analogues was observed.

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Samples: Tissue homogenates, cell lysates and other biological fluids. Assay Type: Quantitative Sandwich. Detection Range: 0.312-20ng/mL. Sensitivity: <0.121ng/mL.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level TRPV1 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level TRPV1 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%

Signal transduction

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of TRPV1 in human tissue homogenates, cell lysates and other biological fluids. Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to TRPV1. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to TRPV1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain TRPV1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of TRPV1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

74315354

NP_542437.2

NM_080706.3

Q8NER1

94,211 Da

Inflammatory Mediator Regulation Of TRP Channels Pathway (948277); Inflammatory Mediator Regulation Of TRP Channels Pathway (948307); Ion Channel Transport Pathway (187193); Neuroactive Ligand-receptor Interaction Pathway (83053); Neuroactive Ligand-receptor Interaction Pathway (462); Stimuli-sensing Channels Pathway (771568); TRP Channels Pathway (906028); Transmembrane Transport Of Small Molecules Pathway (106572); Trk Receptor Signaling Mediated By PI3K And PLC-gamma Pathway (137967); Trk Receptor Signaling Mediated By The MAPK Pathway (138044)

Capsaicin, the main pungent ingredient in hot chili peppers, elicits a sensation of burning pain by selectively activating sensory neurons that convey information about noxious stimuli to the central nervous system. The protein encoded by this gene is a receptor for capsaicin and is a non-selective cation channel that is structurally related to members of the TRP family of ion channels. This receptor is also activated by increases in temperature in the noxious range, suggesting that it functions as a transducer of painful thermal stimuli in vivo. Four transcript variants encoding the same protein, but with different 5' UTR sequence, have been described for this gene. [provided by RefSeq, Jul 2008]

TRPV1: a receptor for capsaicin and a non-selective cation channel that is structurally related to members of the TRP family of ion channels. Activated by increases in temperature in the noxious range, suggesting that it functions as a transducer of painful thermal stimuli in vivo. Protein type: Membrane protein, multi-pass; Receptor, misc.; Membrane protein, integral; Channel, cation. Chromosomal Location of Human Ortholog: 17p13.2. Cellular Component: postsynaptic membrane; integral to plasma membrane; plasma membrane; integral to membrane; cell junction; intrinsic to plasma membrane. Molecular Function: calmodulin binding; transmembrane receptor activity; excitatory extracellular ligand-gated ion channel activity; calcium-release channel activity; calcium channel activity; phosphoprotein binding; ATP binding. Biological Process: cell surface receptor linked signal transduction; transport; chemosensory behavior; thermoception; transmembrane transport

24-Strip-Wells

270 USD

48-Strip-Wells

495

96-Strip-Wells

665

5x96-Strip-Wells

2715

10x96-Strip-Wells

4895