Interleukin 19 (IL19) ELISA Kit | MyBioSource MBS2019896 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Interleukin 19 (IL19) ELISA Kit

catalog :

MBS2019896

quantity :

48-Strip-Wells

price :

540 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS2019896

products type :

ELISA Kit

products full name :

Interleukin 19 (IL19) ELISA Kit

products short name :

Interleukin 19 (IL19)

products name syn :

MDA1; ZMDA1, IL-10C, NG.1; Melanoma Differentiation Associated Protein-Like Protein

other names :

interleukin 19; Interleukin-19; interleukin-19; interleukin 19; Melanoma differentiation-associated protein-like protein; NG.1

products gene name :

IL19

other gene names :

IL19; IL19; MDA1; NG.1; ZMDA1; IL-10C; ZMDA1; IL-19

uniprot entry name :

IL19_HUMAN

reactivity :

Human

sequence length :

215

specificity :

This assay has high sensitivity and excellent specificity for detection of Interleukin 19 (IL19). No significant cross-reactivity or interference between Interleukin 19 (IL19) and analogues was observed.

storage stability :

For unopened kit: All the reagents should be kept according to the labels on vials. The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 degree C upon receipt while the others should be at 4 degree C. For opened kit: When the kit is opened, the remaining reagents still need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and reseal along entire edge of zip-seal. The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

other info1 :

Samples: Serum, plasma, tissue homogenates, Cell lysates, cell culture supernates and other biological fluids. Assay Type: Sandwich. Detection Range: 15.63-1000pg/mL. Sensitivity: Typically less than 5.5pg/mL.

other info2 :

Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Interleukin 19 (IL19) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Interleukin 19 (IL19) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 . Intra-Assay: CV<10% . Inter-Assay: CV<12% . Assay Procedure Summary: 1. Prepare all reagents, samples and standards;. 2. Add 100uL standard or sample to each well. Incubate 2 hours at 37 degree C;. 3. Aspirate and add 100uL prepared Detection Reagent A. Incubate 1 hour at 37 degree C;. 4. Aspirate and wash 3 times;. 5. Add 100uL prepared Detection Reagent B. Incubate 30 minutes at 37 degree C;. 6. Aspirate and wash 5 times;. 7. Add 90uL Substrate Solution. Incubate 15-25 minutes at 37 degree C;. 8. Add 50uL Stop Solution. Read at 450nm immediately.

products description :

Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interleukin 19 (IL19). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Interleukin 19 (IL19). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Interleukin 19 (IL19), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Interleukin 19 (IL19) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

ncbi gi num :

15266466

ncbi acc num :

AAK91776.1

uniprot acc num :

Q9UHD0

ncbi mol weight :

23,079 Da

ncbi pathways :

Cytokine-cytokine Receptor Interaction Pathway (83051); Cytokine-cytokine Receptor Interaction Pathway (460); IL23-mediated Signaling Events Pathway (138000); Jak-STAT Signaling Pathway (83077); Jak-STAT Signaling Pathway (488)

ncbi summary :

The protein encoded by this gene is a cytokine that belongs to the IL10 cytokine subfamily. This cytokine is found to be preferentially expressed in monocytes. It can bind the IL20 receptor complex and lead to the activation of the signal transducer and activator of transcription 3 (STAT3). A similar cytokine in mouse is reported to up-regulate the expression of IL6 and TNF-alpha and induce apoptosis, which suggests a role of this cytokine in inflammatory responses. Alternatively spliced transcript variants encoding the distinct isoforms have been described. [provided by RefSeq, Jul 2008]

uniprot summary :

IL19: May play some important roles in inflammatory responses. Up-regulates IL-6 and TNF-alpha and induces apoptosis. Belongs to the IL-10 family. Protein type: Cytokine; Secreted, signal peptide; Secreted; Apoptosis. Chromosomal Location of Human Ortholog: 1q32.2. Cellular Component: extracellular space; extracellular region. Molecular Function: cytokine activity. Biological Process: apoptosis; positive regulation of JAK-STAT cascade; immune response; signal transduction; inflammatory response; interleukin-6 biosynthetic process

size1 :

48-Strip-Wells

price1 :

540 USD

size2 :

96-Strip-Wells

price2 :

730

size3 :

5x96-Strip-Wells

price3 :

2830

size4 :

10x96-Strip-Wells

price4 :

5080

more info or order :

MyBioSource product webpage