Leukocyte Cell Derived Chemotaxin 2 (LECT2) ELISA Kit | MyBioSource MBS2019828 product information

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product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Leukocyte Cell Derived Chemotaxin 2 (LECT2) ELISA Kit

catalog :

MBS2019828

quantity :

24-Strip-Wells

price :

270 USD

product information

catalog number :

MBS2019828

products type :

ELISA Kit

products full name :

Leukocyte Cell Derived Chemotaxin 2 (LECT2) ELISA Kit

products short name :

[Leukocyte Cell Derived Chemotaxin 2 (LECT2)]

products name syn :

[Chm-II; Chm2]

other names :

[leukocyte cell-derived chemotaxin-2; Leukocyte cell-derived chemotaxin-2; leukocyte cell-derived chemotaxin-2; leukocyte cell-derived chemotaxin 2]

products gene name :

[LECT2]

other gene names :

[LECT2; LECT2; chm2; chm-II; LECT-2; hLECT2]

uniprot entry name :

LECT2_HUMAN

reactivity :

Mouse

sequence length :

151

specificity :

This assay has high sensitivity and excellent specificity for detection of LECT2. No significant cross-reactivity or interference between LECT2 and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between LECT2 and all the analogues, therefore, cross reaction may still exist.

storage stability :

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

image1 heading :

Typical Testing Data/Standard Curve (for reference only)

other info1 :

Samples: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids. Assay Type: Quantitative Sandwich. Detection Range: 78-5,000pg/mL. Sensitivity: 32pg/mL

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level LECT2 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level LECT2 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%

products categories :

Infection immunity

products description :

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of LECT2 in mouse serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids. Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to LECT2. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to LECT2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain LECT2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of LECT2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

ncbi gi num :

59806345

ncbi acc num :

NP_002293.2

ncbi gb acc num :

NM_002302.2

uniprot acc num :

O14960

ncbi mol weight :

16,390 Da

ncbi pathways :

C-MYB Transcription Factor Network Pathway (138073)

ncbi summary :

This gene encodes a secreted, 16 kDa protein that acts as a chemotactic factor to neutrophils and stimulates the growth of chondrocytes and osteoblasts. This protein has high sequence similarity to the chondromodulin repeat regions of the chicken myb-induced myeloid 1 protein. A polymorphism in this gene may be associated with rheumatoid arthritis. [provided by RefSeq, Jul 2008]

uniprot summary :

LECT2: Has a neutrophil chemotactic activity. Also a positive regulator of chondrocyte proliferation. Belongs to the LECT2/MIM-1 family. Protein type: Secreted; Secreted, signal peptide; Motility/polarity/chemotaxis. Chromosomal Location of Human Ortholog: 5q31.1. Cellular Component: extracellular space; cytoplasm. Molecular Function: identical protein binding. Biological Process: negative regulation of Wnt receptor signaling pathway; chemotaxis; skeletal development

size1 :

24-Strip-Wells

price1 :

270 USD

size2 :

48-Strip-Wells

price2 :

505

size3 :

96-Strip-Wells

price3 :

685

size4 :

5x96-Strip-Wells

price4 :

2770

size5 :

10x96-Strip-Wells

price5 :

5025