MyBioSource

ELISA/assay

ELISA Kit for Tyrosine Hydroxylase (TH)

MBS2019774

96 Strip Wells

765 USD

MBS2019774

ELISA Kit

ELISA Kit for Tyrosine Hydroxylase (TH)

Tyrosine Hydroxylase (TH)

tyrosine hydroxylase, partial; Tyrosine 3-monooxygenase; tyrosine 3-monooxygenase; dystonia 14; tyrosine 3-hydroxylase; tyrosine hydroxylase; Tyrosine 3-hydroxylase; TH

TH

TH; TH; TYH; DYT14; DYT5b; TYH; TH

TY3H_HUMAN

Mouse

106

For unopened kit, all the reagents should be kept according to the label on each vial. The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 degree C while others should be at 4 degree C.

Detection Range: 0.156-10ng/mL The standard curve concentrations used for the ELISA’s were 10ng/mL, 5ng/mL, 2.5ng/mL, 1.25ng/mL, 0.625ng/mL, 0.313ng/mL, 0.156ng/mL. Sensitivity: 0.066ng/mL

Assay Type: Sandwich. Sample Type: Serum, plasma, tissue homogenates and other biological fluids.

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Tyrosine Hydroxylase (TH). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Tyrosine Hydroxylase (TH). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Tyrosine Hydroxylase (TH), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Tyrosine Hydroxylase (TH) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

986909

AAA77650.1

44,898 Da

ATF-2 Transcription Factor Network Pathway 138006!!Alcoholism Pathway 585563!!Alcoholism Pathway 587116!!Alpha-synuclein Signaling Pathway 137913!!Amine-derived Hormones Pathway 160983!!Amphetamine Addiction Pathway 547607!!Amphetamine Addiction Pathway 550546!!Biogenic Amine Synthesis Pathway 198793!!Catecholamine Biosynthesis Pathway 160984!!Catecholamine Biosynthesis, Tyrosine = Dopamine = Noradrenaline = Adrenaline Pathway 413357

The protein encoded by this gene is involved in the conversion of tyrosine to dopamine. It is the rate-limiting enzyme in the synthesis of catecholamines, hence plays a key role in the physiology of adrenergic neurons. Mutations in this gene have been associated with autosomal recessive Segawa syndrome. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene. [provided by RefSeq, Jul 2008]

96 Strip Wells

765 USD