ELISA/assay

Ceramide Kinase (CERK) ELISA Kit

MBS2019721

48-Strip-Wells

565 USD

ELISA Kit

Ceramide Kinase (CERK) ELISA Kit

Ceramide Kinase (CERK)

LK4; hCERK; Lipid kinase 4; Acylsphingosine kinase

ceramide kinase; Ceramide kinase; ceramide kinase; ceramide kinase; Acylsphingosine kinase; Lipid kinase 4; LK4

CERK

CERK; CERK; LK4; hCERK; dA59H18.2; dA59H18.3; KIAA1646; hCERK; LK4

CERK1_HUMAN

Human

537

This assay has high sensitivity and excellent specificity for detection of Ceramide Kinase (CERK). No significant cross-reactivity or interference between Ceramide Kinase (CERK) and analogues was observed.

For unopened kit: All the reagents should be kept according to the labels on vials. The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 degree C upon receipt while the others should be at 4 degree C. For opened kit: When the kit is opened, the remaining reagents still need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and reseal along entire edge of zip-seal. The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Samples: Tissue homogenates, Cell lysates and other biological fluids. Assay Type: Sandwich. Detection Range: 3.13-200ng/mL. Sensitivity: Typically less than 1.17ng/mL.

Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Ceramide Kinase (CERK) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Ceramide Kinase (CERK) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 . Intra-Assay: CV<10% . Inter-Assay: CV<12% . Assay Procedure Summary: 1. Prepare all reagents, samples and standards;. 2. Add 100uL standard or sample to each well. Incubate 2 hours at 37 degree C;. 3. Aspirate and add 100uL prepared Detection Reagent A. Incubate 1 hour at 37 degree C;. 4. Aspirate and wash 3 times;. 5. Add 100uL prepared Detection Reagent B. Incubate 30 minutes at 37 degree C;. 6. Aspirate and wash 5 times;. 7. Add 90uL Substrate Solution. Incubate 15-25 minutes at 37 degree C;. 8. Add 50uL Stop Solution. Read at 450nm immediately.

Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Ceramide Kinase (CERK). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Ceramide Kinase (CERK). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Ceramide Kinase (CERK), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Ceramide Kinase (CERK) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

20336726

NP_073603.2

NM_022766.5

Q8TCT0

37,781 Da

Glycosphingolipid Metabolism Pathway (530751); Integrated Breast Cancer Pathway (219801); Metabolism Pathway (477135); Metabolism Of Lipids And Lipoproteins Pathway (160976); Orphan Transporters Pathway (1127546); Sphingolipid Metabolism Pathway (760636); Sphingolipid Metabolism Pathway (82994); Sphingolipid Metabolism Pathway (119543); Sphingolipid Metabolism Pathway (369); Transmembrane Transport Of Small Molecules Pathway (106572)

CERK converts ceramide to ceramide 1-phosphate (C1P), a sphingolipid metabolite. Both CERK and C1P have been implicated in various cellular processes, including proliferation, apoptosis, phagocytosis, and inflammation (Kim et al., 2006 [PubMed 16488390]).[supplied by OMIM, Mar 2008]

CERK: Catalyzes specifically the phosphorylation of ceramide to form ceramide 1-phosphate. Acts efficiently on natural and analog ceramides (C6, C8, C16 ceramides, and C8-dihydroceramide), to a lesser extent on C2-ceramide and C6-dihydroceramide, but not on other lipids, such as various sphingosines. Binds phosphoinositides. Protein type: Lipid Metabolism - sphingolipid; Kinase, lipid; EC 2.7.1.138. Chromosomal Location of Human Ortholog: 22q13.31. Cellular Component: mitochondrion; plasma membrane; integral to membrane. Molecular Function: ceramide kinase activity; magnesium ion binding; diacylglycerol kinase activity; ATP binding; NAD+ kinase activity. Biological Process: sphingolipid metabolic process; protein kinase C activation; glycosphingolipid metabolic process; ceramide metabolic process; lipid phosphorylation; transmembrane transport

48-Strip-Wells

565 USD

96-Strip-Wells

765

5x96-Strip-Wells

2975

10x96-Strip-Wells

5340